Specific Detection of Alpha-Fetoprotein Using AlGaAs/GaAs High Electron Mobility Transistors
Specific Detection of Alpha-Fetoprotein Using AlGaAs/GaAs High Electron Mobility Transistors
复制标题
DOI：
10.1109/led.2014.2298397
复制
发表时间：
2014-01
影响因子：
4.9
通讯作者：
K. Ding;Cheng-yang Wang;Bintian Zhang;Yang Zhang;Min Guan;L. Cui;Yuwei Zhang;Yiping Zeng;Zhang Lin;F. Huang
中科院分区：
文献类型：
--
作者：
K. Ding;Cheng-yang Wang;Bintian Zhang;Yang Zhang;Min Guan;L. Cui;Yuwei Zhang;Yiping Zeng;Zhang Lin;F. Huang
甲胎蛋白（AFP）是早期诊断典型的肿瘤标志物。利用AlGaAs/GaAs高电子迁移率晶体管（hemt）对AFP进行了高特异性检测。用共价修饰法将抗afp抗体固定在HEMT的au包被门区。为了避免任何非特异性结合，在羰基还原后，该门被牛血清白蛋白阻断。AFP的检测限低至几皮克/毫升，并且传感器的交叉反应性可以忽略不计，表明其高选择性。微量样品的成功检测表明该传感器在广泛人群的实际筛选中具有很大的潜力。
Alpha-fetoprotein (AFP) is a typical tumor marker in early diagnosis. Highly specific detection of the AFP was demonstrated using AlGaAs/GaAs high electron mobility transistors (HEMTs). Anti-AFP antibody was immobilized to the Au-coated gate area of the HEMT by a covalent modification method. To avoid any nonspecific binding, the gate was blocked by bovine serum albumin after the reduction of the carbonyl group. A detection limit for the AFP as low as a few picograms/milliliter was achieved, and cross reactivity of the sensor was found negligible, demonstrating its high selectivity. Successful detection with minute quantity of the sample indicates that the sensor has great potential in practical screening for a wide population.