Role of jasmonates in the elicitor- and wound-inducible expression of defense genes in parsley and transgenic tobacco
Role of jasmonates in the elicitor- and wound-inducible expression of defense genes in parsley and transgenic tobacco
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DOI：
10.1104/pp.112.1.183
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发表时间：
1996-09-01
期刊：
影响因子：
7.4
通讯作者：
Douglas, CJ
中科院分区：
文献类型：
--
作者：
EllardIvey, M;Douglas, CJ
茉莉酸类化合物被认为是植物损伤和/或激发子激活的防御基因表达的信号中间体。我们使用欧芹（岩芹）细胞培养物和转基因烟草（烟草）植物表达4CL 1-CUS基因融合研究茉莉酸酯介导的创伤和/或诱导子激活苯丙素和其他防御相关基因的潜在作用。茉莉酸酯和α-亚麻酸强烈诱导4CL的表达在欧芹细胞中的剂量依赖性的方式;茉莉酸甲酯也激活了其他苯丙素类基因的协调表达和呋喃香豆素植物抗毒素的积累。然而，最佳茉莉酸甲酯浓度的细胞的反应是不同的定量和定性的激发子处理的细胞的反应。在转基因烟草中，创伤诱导型烟草4CL基因和4CL 1启动子-GUS转基因以剂量依赖的方式响应于茉莉酸和α-亚麻酸。用脂氧合酶抑制剂预处理欧芹细胞或烟叶，降低它们对激发子和创伤的反应。这些结果表明，在欧芹细胞中的激发子反应可以部分地模仿茉莉酸酯处理，这支持茉莉酸酯在介导4CL和其他苯丙素类基因的伤口诱导的表达的作用。
Jasmonates have been proposed to be signaling intermediates in the wound and/or elicitor-activated expression of plant defense genes. We used parsley (Petroselinum crispum) cell cultures and transgenic tobacco (Nicotiana tabacum) plants expressing 4CL1-CUS gene fusions to investigate the potential role played by jasmonates in mediating the wound and/or elicitor activation of phenylpropanoid and other defense-related genes. Jasmonates and alpha-linolenic acid strongly induced the expression of 4CL in a dose-dependent manner in parsley cells; methyl jasmonate also activated the coordinate expression of other phenylpropanoid genes and the accumulation of furanocoumarin phytoalexins. However, the response of the cells to optimal methyl jasmonate concentrations was distinct quantitatively and qualitatively from the response of elicitor-treated cells. In transgenic tobacco wound-inducible tobacco 4CL genes and a 4CL1 promoter-GUS transgene were responsive to jasmontes and alpha-linolenic acid in a dose-dependent manner. Pretreatment of parsley cells or tobacco leaves with a lipoxygenase inhibitor reduced their responsiveness to the elicitor and to wounding. These results show that the elicitor response in parsley cells can be partially mimicked by jasmonate treatment, which supports a role for jasmonates in mediating wound-induced expression of 4CL and other phenylpropanoid genes.