Transgenic Zebrafish Expressing Green Fluorescent Protein in Dopaminergic Neurons of the Ventral Diencephalon
Transgenic Zebrafish Expressing Green Fluorescent Protein in Dopaminergic Neurons of the Ventral Diencephalon
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DOI：
10.1002/dvdy.22742
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发表时间：
2011-11-01
影响因子：
2.5
通讯作者：
Ekker, Marc
中科院分区：
文献类型：
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作者：
Xi, Yanwei;Yu, Man;Ekker, Marc
我们已经产生了一个线的转基因斑马鱼，Tg（DAT：EGFP），其中的绿色荧光蛋白（GFP）的多巴胺转运蛋白（DAT）基因的顺式调控元件的控制下表达。在Tg（日期：在GFP）鱼中，多巴胺（DA）神经元用GFP标记，包括腹侧间脑（vDC）簇中的多巴胺（DA）神经元、视网膜中的无长突细胞、嗅球中的无长突细胞、前顶盖中的无长突细胞和尾侧下丘脑中的多巴胺（DA）神经元。在vDC中，基于共定位分析，组2-6的DA神经元被GFP正确标记。MPTP（1-甲基-4-苯基-1，2，3，6-四氢吡啶）处理在vDC的第2-6组中诱导了适度但显著的DA神经元损失。该转基因株系将有助于DA神经元发育的研究和DA神经元丢失的模型。Developmental Dynamics 240：2539-2547，2011. (C)2011 Wiley Periodicals，Inc.
We have generated a line of transgenic zebrafish, Tg(dat:EGFP), in which the green fluorescent protein (GFP) is expressed under the control of cis-regulatory elements of the dopamine transporter (dat) gene. In Tg(dat: EGFP) fish, dopamine (DA) neurons are labeled with GFP, including those in ventral diencephalon (vDC) clusters, amacrine cells in the retina, in the olfactory bulb, in the pretectum, and in the caudal hypothalamus. In the vDC, DA neurons of groups 2-6 are correctly labeled with GFP, based on colocalization analyses. MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) treatments induced a modest but significant loss of DA neurons in groups 2-6 of the vDC. This transgenic line will be useful for the study of DA neuron development and in models of DA neuron loss. Developmental Dynamics 240: 2539-2547, 2011. (C) 2011 Wiley Periodicals, Inc.