Disruption of non-anchored cell wall protein NCW-1 promotes cellulase production by increasing cellobiose uptake in Neurospora crassa
Disruption of non-anchored cell wall protein NCW-1 promotes cellulase production by increasing cellobiose uptake in Neurospora crassa
复制标题
非锚定细胞壁蛋白 NCW-1 的破坏通过增加粗糙脉孢菌中纤维二糖的摄取来促进纤维素酶的产生
DOI：
10.1007/s10529-016-2274-1
复制
发表时间：
2017-04-01
影响因子：
2.7
通讯作者：
Tian, Chaoguang
中科院分区：
文献类型：
--
作者：
Lin, Liangcai;Chen, Yong;Tian, Chaoguang
目的阐明纤维素酶在丝状真菌中的信号转导机制，包括纤维素酶诱导途径的组成。结果粗糙脉孢菌NCW-1编码一种非锚定的细胞壁蛋白。NCW-1的缺失增加了纤维素酶基因的表达，这不是由于减轻了cre-1途径介导的碳分解代谢抑制。构建了一个缺失三种主要β-葡萄糖苷酶基因和NCW-1(Delta 3βG Delta NCW-1)的突变体。四重突变体的转录组分析显示，NCW-1缺失后纤维糊精转运蛋白的表达增强，表明NCW-1通过抑制纤维糊精的摄取而影响纤维素酶的表达和产量。结论NCW-1是一个在纤维素酶诱导信号通路中发挥关键作用的新成分。
Objectives To elucidate the mechanism of cellulase signal transduction in filamentous fungi including the components of the cellulase induction pathway.Results Neurospora crassa ncw-1 encodes a non-anchored cell wall protein. The absence of ncw-1 increased cellulase gene expression and this is not due to relieving carbon catabolite repression mediated by the cre-1 pathway. A mutant lacking genes encoding both three major beta-glucosidase enzymes and NCW-1 (Delta 3 beta G Delta ncw-1) was constructed. Transcriptome analysis of the quadruple mutant demonstrated enhanced expression of cellodextrin transporters after ncw-1 deletion, indicating that ncw-1 affects cellulase expression and production by inhibiting the uptake of the cellodextrin.Conclusions NCW-1 is a novel component that plays a critical role in the cellulase induction signaling pathway.