Selective isolation of tryptophan-containing peptides by hydrophobicity modulation.
Selective isolation of tryptophan-containing peptides by hydrophobicity modulation.
复制标题
通过疏水性调节选择性分离含色氨酸的肽。
DOI：
10.1016/0003-2697(83)90288-9
复制
发表时间：
1983
影响因子：
2.9
通讯作者：
Walsh,KA
中科院分区：
文献类型：
--
作者：
Sasagawa,T;Titani,K;Walsh,KA
含色氨酸的多肽是通过利用与邻硝基苯硫氯反应引起的疏水性和层析迁移率的变化从复杂的消化液中选择性地分离出来的。在高效液相色谱过程中，通过监测荧光，这些多肽首先被定位在粗馏分中，然后进行化学修饰，以便在随后的重新层析中将它们从污染物中分离出来。
Tryptophan-containing peptides are selectively isolated from complex digests by taking advantage of changes in hydrophobicity and chromatographic mobility induced by reaction with o-nitrophenylsulfenyl chloride. The peptides are first located in crude fractions by monitoring the fluorescence during high-performance liquid chromatography and then chemically modified to facilitate their separation from contaminants during subsequent rechromatography.