Association between bacterial community structures and mortality of fish larvae in intensive rearing systems
Association between bacterial community structures and mortality of fish larvae in intensive rearing systems
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DOI：
10.1111/j.1444-2906.2007.01397.x
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发表时间：
2007-08-01
影响因子：
1.9
通讯作者：
Eguchi, Mitsuru
中科院分区：
文献类型：
--
作者：
Nakase, Gentoku;Nakagawa, Yoshizumi;Eguchi, Mitsuru
采用荧光原位杂交法对养鱼用水中细菌群落结构进行了分析。试验一在两个商品化的育种池中饲养罗非鱼仔鱼。尽管在两个池塘中都加入了浮游植物，但池塘1的存活率高于池塘2。在水池2中，伽玛-蛋白细菌在第13天成为优势菌(相当于总细菌的70%)，此后出现了严重的幼虫死亡。然而，在水池1中，从第1天到第13天，α-蛋白细菌和细胞吞噬-黄杆菌群占主导地位；没有显著的死亡记录。在实验2中，对大理石虎鱼幼体进行了添加或不添加微绿球藻的培养。实验结束时，幼虫在含微绿球藻的水族箱中的存活率。均显著高于对照组(P<；0.05)。在没有微绿球藻的养殖水体中，伽马蛋白细菌在养殖过程中有所增加。在微绿球藻养殖水体中，α-蛋白细菌和细胞吞噬-黄杆菌群落在实验初期占优势，而γ-蛋白细菌的相对丰度在整个实验过程中维持在较低水平。α-蛋白细菌和细胞吞噬-黄杆菌群的优势似乎是成功生产幼虫的一个很好的指标。
Bacterial community structures were analyzed in water used for rearing fish larvae by fluorescence in situ hybridization. In Experiment 1, red sea bream Pagrus major larvae were reared in two commercial seed production tanks. The survival rate in Tank 1 was higher than in Tank 2, even though phytoplankton, Nannochloropsis sp., was added to both tanks. In Tank 2, gamma-proteobacteria became dominant (similar to 70% of total bacteria) on day 13, thereafter heavy larval mortalities occurred. In Tank 1, however, alpha-proteobacteria and the Cytophaga-Flavobacterium cluster were predominant from day -1 until day 13; no significant mortality was recorded. In Experiment 2, marble goby Oxyeleotris marmoratus larvae were cultured with or without Nannochloropsis sp. At the end of the experiment, larval survival rates in aquaria with Nannochloropsis sp. were significantly (P < 0.05) higher than those without. In rearing water without Nannochloropsis sp., gamma-proteobacteria increased during rearing. In rearing water with Nannochloropsis sp., alpha-proteobacteria and the Cytophaga-Flavobacterium cluster were predominant at the beginning of the experiments and the relative abundance of gamma-proteobacteria was maintained at a lower level throughout the experiments. The predominance of alpha-proteobacteria and the Cytophaga-Flavobacterium cluster appears to be a good indicator of successful larval production.