The response of growth and patulin production of postharvest pathogen Penicillium expansum to exogenous potassium phosphite treatment
The response of growth and patulin production of postharvest pathogen Penicillium expansum to exogenous potassium phosphite treatment
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DOI：
10.1016/j.ijfoodmicro.2016.12.017
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
周婷
中科院分区：
文献类型：
--
作者：
赖童飞;汪莹;樊亚亚;周莹莹;鲍莹;周婷
In this study, the effects of exogenous potassium phosphite (Phi) on growth and patulin production of posthar-.vest pathogen Penicillium expansum were assessed. The results indicated that P. expansum under 5 mmol/L Phi.stress presented obvious development retardation, yield reduction of patulin and lower infectivity to apple.fruit. Meanwhile, expression analysis of 15 genes related to patulin biosynthesis suggested that Phi mainly affect-.ed the early steps of patulin synthetic route at transcriptional level. Furthermore, a global view of proteome and.transcriptome alteration of P. expansum spores during 6 h of Phi stress was evaluated by iTRAQ (isobaric tags for.relative and absolute quantitation) and RNA-seq (RNA sequencing) approaches. A total of 582 differentially.expressed proteins (DEPs) and 177 differentially expressed genes (DEGs) were acquired, most of which partici-.pated in carbohydrate metabolism, amino acid metabolism, lipid metabolism, genetic information processing.and biosynthesis of secondary metabolites. Finally, 39 overlapped candidates were screened out through corre-.lational analysis between iTRAQ and RNA-seq datasets. These findings will afford more precise and directional.clues to explore the inhibitory mechanism of Phi on growth and patulin biosynthesis of P. expansum, and be ben-.eficial to develop effective controlling approaches based on Phi.
