Autonomous parvovirus DNA replication requires topoisomerase I and its activity is increased during infection.
Autonomous parvovirus DNA replication requires topoisomerase I and its activity is increased during infection.
复制标题
细小病毒DNA的自主复制需要拓扑异构酶I，并且其活性在感染期间增加。
DOI：
10.1128/jvi.65.3.1662-1665.1991
复制
发表时间：
1991
影响因子：
5.4
通讯作者：
Rhode,SL
中科院分区：
文献类型：
--
作者：
Gu,ML;Rhode,SL
Topoisomerases I and II (topo I and topo II) are nuclear enzymes functioning to resolve DNA topological problems during replication, transcription, and other DNA processes. We tested the effects of camptothecin and VP16, specific inhibitors of topo I and II, respectively, on the DNA replication of parvoviruses LuIII and H-1 and found that viral DNA synthesis was suppressed by camptothecin but not by VP16. Transcription of H-1 virus was measured by a nuclear runoff assay and showed no inhibition by camptothecin. Interestingly, topo I in the LuIII virus-infected cell nuclear extract appears to have more activity for covalently binding to viral DNA than that in mock-infected cell nuclear extracts. Our data suggested that this activity was not due to an increased transcription of the topo I gene or to greater amounts of topo I.
