Metabolic signatures of hepatolithiasis using ultra-high performance liquid chromatography-tandem mass spectrometry
Metabolic signatures of hepatolithiasis using ultra-high performance liquid chromatography-tandem mass spectrometry
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使用超高效液相色谱-串联质谱法分析肝胆管结石的代谢特征
DOI：
10.1007/s11306-022-01927-2
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发表时间：
2022-08
期刊：
影响因子：
3.6
通讯作者：
Wenjun Liao
中科院分区：
文献类型：
--
作者：
Cong Wang;Jun Yang;Enliang Li;Shuaiwu Luo;Chi Sun;Yuting Liao;Min Li;Jin Ge;Jun Lei;Fan Zhou;Linquan Wu;Wenjun Liao
背景与目的肝胆管结石的代谢组学研究尚未完成。本研究的目的是从代谢组学的角度分析肝内胆管结石患者和20例健康人的血清代谢物。方法采用高效液相色谱-串联质谱仪对30例肝内胆管结石患者和20例健康人的血清代谢物进行综合分析。统计分析使用单变量(学生t检验)和多变量(正交偏最小二乘判别分析)统计和R语言。结果共鉴定出277种代谢物，它们在肝胆管结石血清组和健康对照血清组之间存在显著差异。这些代谢物主要是脂类和类脂分子及氨基酸代谢物。肝胆管结石血清组类固醇激素生物合成途径丰富。在所有特异性代谢产物中，肝内胆管结石血清组有75种代谢产物过度表达。60种代谢物的AUC值均大于0.70，其中18-β-甘草次酸、FMH、利福平和PC(4：0/16：2)等4种代谢物的AUC值均大于0.90。共鉴定出60个潜在的代谢标志物，其中18-β-甘草次酸、FMH、利福平和PC(4：0/16：2)可能在肝胆管结石中具有潜在的临床应用价值。
Background & aimsA metabolomic study of hepatolithiasis has yet to be performed. The purpose of the present study was to characterize the metabolite profile and identify potential biomarkers of hepatolithiasis using a metabolomic approach.MethodsWe comprehensively analyzed the serum metabolites from 30 patients with hepatolithiasis and 20 healthy individuals using ultra-high performance liquid chromatography-tandem mass spectrometry operated in negative and positive ionization modes. Statistical analyses were performed using univariate (Student’s t-test) and multivariate (orthogonal partial least-squares discriminant analysis) statistics and R language. Receiver operator characteristic (ROC) curve analysis was performed to identify potential predictors of hepatolithiasis.ResultsWe identified 277 metabolites that were significantly different between hepatolithiasis serum group and healthy control serum group. These metabolites were principally lipids and lipid-like molecules and amino acid metabolites. The steroid hormone biosynthesis pathway was enriched in hepatolithiasis serum group. In all specific metabolites, 75 metabolites were over-expressed in hepatolithiasis serum group. The AUC values for 60 metabolites exceeded 0.70, 4 metabolites including 18-β-Glycyrrhetinic acid, FMH, Rifampicin and PC (4:0/16:2) exceeded 0.90.ConclusionsWe have identified serum metabolites that are associated with hepatolithiasis for the first time. 60 potential metabolic biomarkers were identified, 18-β-Glycyrrhetinic acid, FMH, Rifampicin and PC (4:0/16:2) may have the potential clinical utility in hepatolithiasis.
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DOI：
10.1097/mco.0b013e3283600d46
发表时间：
2013-05
影响因子：
3.1
作者：
Mehedint MG;Zeisel SH
通讯作者：
Zeisel SH
影响因子：
25.7
作者：
McNeilly AD;Macfarlane DP;O'Flaherty E;Livingstone DE;Mitić T;McConnell KM;McKenzie SM;Davies E;Reynolds RM;Thiesson HC;Skøtt O;Walker BR;Andrew R
通讯作者：
Andrew R
影响因子：
6.7
作者：
S. Seijo;J. Lozano;C. Alonso;R. Miquel;A. Berzigotti;E. Reverter;F. Turon;M. Martínez‐Chantar;A. Castro;J. Mato;V. Hernández-Gea;J. Bosch;J. García‐Pagán
通讯作者：
S. Seijo;J. Lozano;C. Alonso;R. Miquel;A. Berzigotti;E. Reverter;F. Turon;M. Martínez‐Chantar;A. Castro;J. Mato;V. Hernández-Gea;J. Bosch;J. García‐Pagán
影响因子：
--
作者：
Liu Z;Tian F;Feng X;He Y;Jiang P;Li J;Guo F;Zhao X;Chang H;Wang S
通讯作者：
Wang S
DOI：
10.1002/hep.30319
发表时间：
2019-08
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Banales JM;Iñarrairaegui M;Arbelaiz A;Milkiewicz P;Muntané J;Muñoz-Bellvis L;La Casta A;Gonzalez LM;Arretxe E;Alonso C;Martínez-Arranz I;Lapitz A;Santos-Laso A;Avila MA;Martínez-Chantar ML;Bujanda L;Marin JJG;Sangro B;Macias RIR
通讯作者：
Macias RIR