Heat stress-induced transposon activation correlates with 3D chromatin organization rearrangement in Arabidopsis
Heat stress-induced transposon activation correlates with 3D chromatin organization rearrangement in Arabidopsis
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DOI：
10.1038/s41467-020-15809-5
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发表时间：
2020-04
影响因子：
16.6
通讯作者：
Linhua Sun;Yuqing Jing;Xinyu Liu;Qi Li;Z. Xue;Zhukuan Cheng;Daowen Wang;Hang He;Weiqiang Qian
中科院分区：
文献类型：
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作者：
Linhua Sun;Yuqing Jing;Xinyu Liu;Qi Li;Z. Xue;Zhukuan Cheng;Daowen Wang;Hang He;Weiqiang Qian
在高等真核生物中，异染色质主要由被表观遗传机制沉默的转座因子（te）组成。但是，某些异染色质相关te的沉默被热应激破坏。通过比较在正常或高温条件下获得的全基因组高分辨率染色质包装模式，我们在这里表明，热应激导致拟南芥三维基因组的全局重排。中心点周围区域和远端染色体臂之间的接触，以及近端染色体内沿染色体的相互作用，都得到加强。然而，胞心粒内的相互作用和远端染色体内区域之间的相互作用减少。许多染色体间的相互作用，包括结内的相互作用，也减少了。此外，te的热活化表现出与染色体相互作用的减少高度相关，这些相互作用涉及到被活化的te的周围粒、结、旋钮以及上游和下游侧翼区域。总之，我们的研究结果为TE激活和3D基因组重组之间的关系提供了见解。
In higher eukaryotes, heterochromatin is mainly composed of transposable elements (TEs) silenced by epigenetic mechanisms. But, the silencing of certain heterochromatin-associated TEs is disrupted by heat stress. By comparing genome-wide high-resolution chromatin packing patterns under normal or heat conditions obtained through Hi-C analysis, we show here that heat stress causes global rearrangement of the 3D genome inArabidopsis thaliana. Contacts between pericentromeric regions and distal chromosome arms, as well as proximal intra-chromosomal interactions along the chromosomes, are enhanced. However, interactions within pericentromeres and those between distal intra-chromosomal regions are decreased. Many inter-chromosomal interactions, including those within theKNOT, are also reduced. Furthermore, heat activation of TEs exhibits a high correlation with the reduction of chromosomal interactions involving pericentromeres, theKNOT, the knob, and the upstream and downstream flanking regions of the activated TEs. Together, our results provide insights into the relationship between TE activation and 3D genome reorganization.