Expression changes of K+-Cl- co-transporter 2 and Na+-K+-Cl- co-transporter1 in mouse trigeminal subnucleus caudalis following pulpal inflammation
Expression changes of K+-Cl- co-transporter 2 and Na+-K+-Cl- co-transporter1 in mouse trigeminal subnucleus caudalis following pulpal inflammation
复制标题
牙髓炎症后小鼠三叉神经尾亚核K-Cl-协同转运蛋白2和Na-K-Cl-协同转运蛋白1的表达变化
DOI：
10.1016/j.brainresbull.2010.01.002
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发表时间：
2010-04-05
影响因子：
3.8
通讯作者：
Wu, Li-an
中科院分区：
文献类型：
--
作者：
Li, Yong-Qi;Li, Hongshi;Wu, Li-an
阳离子氯共转运体，包括K+-Cl-共转运体2（KCC 2）和Na+-K+-Cl-共转运体I（NKCC 1），对GABA能传递特别重要，因此参与脊髓水平的痛觉过敏的发展。然而，三叉神经系统中的这些共转运蛋白是否会导致牙痛，在很大程度上还不清楚。在这项研究中，我们研究了KCC 2和NKCC 1 mRNA的表达在小鼠三叉神经尾侧亚核（Vc）后脂多糖（LPS）应用于牙髓实时逆转录聚合酶链反应（RT-PCR）方法。牙髓炎症后1 d，KCC 2 mRNA表达下调，NKCC 1 mRNA表达上调。用K252 a阻断内源性脑源性神经营养因子-酪氨酸受体激酶B通路可产生明显的抗伤害感受作用，这一点可通过LPS处理小鼠的伸舌行为减少来证明。这些数据表明，Vc中的KCC 2和NKCC 1可能在牙髓炎症过程中的伤害性感受和牙痛的传递中起关键作用。(C)2010年爱思唯尔公司All rights reserved.
Cation chloride co-transporters, including K+-Cl- co-transporter 2 (KCC2) and Na+-K+-Cl- co-transporter I (NKCC1), are of particular importance to GABAergic transmission and thus involved in the development of hyperalgesia at the spinal level. However, it is largely unknown whether these co-transporters in the trigeminal system contribute to dental pain. In this study, we investigated the expression of KCC2 and NKCC1 mRNAs in mouse trigeminal subnucleus caudalis (Vc) after lipopolysaccharide (LPS) application to the tooth pulp by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method. KCC2 mRNA was found to be down-regulated at 1 d after pulpal inflammation, while NKCC1 was up-regulated. Blockade of endogenous brain-derived neurotrophic factor-tyrosine receptor kinase B pathway with K252a produced pronounced antinociception as evidenced by decreased tongue protrusion behavior in LPS-treated mice. These data suggest that KCC2 and NKCC1 in Vc may play a critical role in the nociception and transmission of dental pain during pulpal inflammation. (C) 2010 Elsevier Inc. All rights reserved.