Toll-like receptor expression in feline lymphoid tissues
Toll-like receptor expression in feline lymphoid tissues
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DOI：
10.1016/j.vetimm.2005.02.022
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发表时间：
2005-07-15
影响因子：
1.8
通讯作者：
Dean, GA
中科院分区：
文献类型：
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作者：
Ignacio, G;Nordone, S;Dean, GA
Toll 样受体 (TLR) 是种系编码的模式识别受体 (PRR)，可激活先天免疫系统。虽然很明显 TLR 在针对病原体的免疫反应中很重要，但它们也可能被某些病原体利用。我们的目标是确定猫免疫缺陷病毒 (FIV) 感染是否会影响 TLR 表达或功能，从而导致先天免疫功能障碍。为此，我们克隆了猫科动物 TLR 1-3、5-8 的部分序列，并开发了实时 PCR 测定法来量化猫科动物 TLR 1-9。对正常猫淋巴组织、纯化的淋巴细胞亚群和 FIV 感染的细胞系中的 TLR 表达进行了定量。 TLRs在脾脏、肠系膜淋巴结、咽后淋巴结、胸腺、肠上皮内淋巴细胞和固有层淋巴细胞中存在不同的表达模式。 B淋巴细胞、CD4+ T细胞和CD8+ T细胞均表达TLR 2-5、7-9；然而，不同淋巴细胞表型的相对表达水平有所不同。 FIV 感染细胞系会导致 TLR 表达水平发生改变，其表达水平因细胞类型而异。这些结果表明，TLR 的组织分布与特定组织的免疫作用相关，淋巴细胞也可能表达这些“先天免疫”受体，并且 FIV 感染可以改变 TLR 的表达。 (c) 2005 年 Elsevier B.V. 出版
Toll-like receptors (TLRs) are germline-encoded pattern recognition receptors (PRRs) that activate the innate immune system. While it is clear that TLRs are important in the immune response against pathogens, they may also be exploited by some pathogens. Our objective is to determine whether feline immunodeficiency virus (FIV) infection affects TLR expression or function thereby resulting in innate immune dysfunction. To this end, we cloned partial sequences for feline TLRs 1-3, 5-8, and developed real-time PCR assays to quantify feline TLRs 1-9. TLR expression was quantified in normal cat lymphoid tissues, purified lymphocyte subsets, and FIV-infected cell lines. Different expression patterns of TLRs were found in spleen, mesenteric lymph node, retropharyngeal lymph node, thymus, intestinal intraepithelial lymphocytes, and lamina propria lymphocytes. B lymphocytes, CD4+ T cells, and CD8+ T cells all expressed TLRs 2-5, 7-9; however, the relative levels of expression varied among lymphocyte phenotypes. Infection of cell lines with FIV resulted in altered TLR expression levels that differed depending on cell type. These results demonstrate that tissue distribution of TLRs is associated with the immunological role of a particular tissue, that lymphocytes may also express these 'innate immune' receptors, and that FIV infection can alter TLR expression. (c) 2005 Published by Elsevier B.V.