Establishment of Mice Expressing EGFP in the Placode-Derived Inner Ear Sensory Cell Lineage and FACS-Array Analysis Focused on the Regional Specificity of the Otocyst
Establishment of Mice Expressing EGFP in the Placode-Derived Inner Ear Sensory Cell Lineage and FACS-Array Analysis Focused on the Regional Specificity of the Otocyst
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DOI：
10.1002/cne.22468
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发表时间：
2010-12-01
影响因子：
2.5
通讯作者：
Kurihara, Hiroki
中科院分区：
文献类型：
--
作者：
Fujimoto, Chisato;Ozeki, Hidenori;Kurihara, Hiroki
在这项研究中，我们建立了一个新的增强型绿色荧光蛋白（EGFP）报告小鼠系，使基板来源的内耳感觉细胞谱系的可视化EGFP最初表达在耳基板和整个分化过程中的内耳感觉补丁在胚胎105天（E10 5），EGFP在耳囊腹侧和背内侧区表达，这些区域主要产生耳蜗，包括Corti器和球囊，包括黄斑和内淋巴管。该区域还可以产生将发育为前感觉细胞或平衡听神经节成神经细胞的细胞。通过使用该线和荧光激活细胞分选（FACS）阵列技术，我们开发了耳囊EGFP阳性区域的区域特异性的新基因表达谱，包括Otx 1阳性区域，结果表明，芯片数据的信号对数比在预测主要在腹侧和/或腹侧表达的基因方面具有较高的效率。或背内侧耳囊肿，并且数据可以被挖掘以揭示许多涉及内耳形态发生和细胞命运调节的新基因。此外，这些数据表明，一些新的基因富集在绿色荧光蛋白-阳性区域可能与人类先天性感音神经性听力损失有关。在发育中的内耳中的区域特异性基因网络和新基因功能的鉴定J Comp Neurol 518 4702-4722，2010（C）2010 Wiley利斯公司
In this study, we established a novel enhanced green fluorescent protein (EGFP) reporter mouse line that enables the visualization of the placode-derived inner ear sensory cell lineage EGFP was initially expressed in the otic placode and throughout its differentiation process into the inner ear sensory patches At embryonic day 105 (E10 5), EGFP was expressed in the ventral and dorsomedial region of the otocyst These regions could mainly give rise to the cochlea, including the organ of Corti, and the saccule, including the macula and the endolymphatic duct The region could also give rise to cells that will develop as either prosensory cells or statoacoustic ganglion neuroblasts By using this line and fluorescence-activated cell sorting (FACS)-array technology, we developed a new gene expression profile of the regional specificity of the otocyst EGFP-positive regions include the Otx1-positive region, which could be clearly distinguished from EGFP-negative regions The signal log ratio of microarray data showed high efficiency in predicting the genes expressed mainly in the ventral and/or dorsomedial otocyst and the data could be mined to uncover many novel genes involved in inner ear morphogenesis and cell fate regulation Additionally, these data suggest that some novel genes enriched in EGFP-positive regions may be potentially involved in human congenital sensorineural hearing loss This reporter line could play important roles in the use of animal models for detailed analysis of the differentiation process into the sensory patches and the identification of regional-specific gene networks and novel gene functions in the developing inner ear J Comp Neurol 518 4702-4722, 2010 (C) 2010 Wiley Liss Inc