PROTEIN-MODIFIED ELECTRODES - THE GLUCOSE-OXIDASE POLYPYRROLE SYSTEM
PROTEIN-MODIFIED ELECTRODES - THE GLUCOSE-OXIDASE POLYPYRROLE SYSTEM
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DOI：
10.1021/ac00127a018
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发表时间：
1986-12-01
影响因子：
7.4
通讯作者：
WALLER, J
中科院分区：
文献类型：
--
作者：
UMANA, M;WALLER, J
本文报道了一种新的方法，电极固定化酶，葡萄糖氧化酶，吡咯在酶的存在下，通过电聚合。酶被生长在电极表面上的聚吡咯膜捕获。由于聚吡咯是一种电子导电的有机聚合物，其作用是将酶包裹在导电电极中。此外，酶与葡萄糖反应的产物在紧邻该导电材料的地方产生。本研究包括（1）在与酶固定相容的条件下制备和表征聚吡咯修饰电极，（2）证明聚吡咯修饰电极相对稳定并且适用于间接测定在接触电极的溶液中发生的葡萄糖氧化酶催化反应的电化学活性产物，和（3）证明葡萄糖氧化酶被固定到薄聚吡咯膜中，在酶存在下电聚合到表面上。电聚合的酶是活性的，并作为葡萄糖电极系统的基础，将保持活性数天。
This paper reports a novel approach to electrode immobilization of an enzyme, glucose oxidase, by electropolymerization of pyrrole in the presence of the enzyme. The enzyme becomes entrapped by the polypyrrole film growing on the electrode surface. Since polypyrrole is an electronically conducting organic polymer, the effect is to enfold the enzyme in a conducting electrode. In addition, the products of the enzyme reaction with glucose are produced in the immediate proximity of this conducting material. This study included (1) preparation and characterization of polypyrrole-modified electrodes under conditions compatible with enzyme immobilization, (2) demonstration that polypyrrole-modified electrodes are relatively stable and applicable for the indirect determination of electrochemically active products of glucose oxidase catalyzed reactions that occur in a solution contacting the electrode, and (3) demonstration that glucose oxidase becomes immobilized into a thin polypyrrole film, electropolymerized onto surfaces in the presence of the enzyme. The electropolymerized enzyme is active and serves as the basis for a glucose electrode system that will remain active for several days.