Single embryo and oocyte lipid fingerprinting by mass spectrometry
Single embryo and oocyte lipid fingerprinting by mass spectrometry
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DOI：
10.1194/jlr.d001768
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发表时间：
2010-05-01
影响因子：
6.5
通讯作者：
Eberlin, Marcos N.
中科院分区：
文献类型：
--
作者：
Ferreira, Christina R.;Saraiva, Sergio A.;Eberlin, Marcos N.
用于胚胎和卵母细胞中脂质分析的方法通常涉及从许多样品池中选择性地提取脂质，然后通过色谱技术进行化学操作、分离和表征单个组分。在这里，我们报告直接分析基质辅助激光解吸/电离质谱（MALDI-MS）的单个和完整的胚胎或卵母细胞从不同的物种。生物样品进行简单的润湿与基质溶液和特征脂质（代表磷脂酰胆碱，鞘磷脂和三酰甘油）的配置文件，通过MALDI-MS获得。作为代表性的例子，人，牛，羊和鱼的卵母细胞，以及牛和昆虫胚胎进行了分析。MALDI-MS显示能够提供配子和胚胎的特征脂质谱，并且还响应于由于牛胚胎的发育阶段和体外培养条件而引起的修饰。因此，胚胎和卵母细胞中结构和储备脂质的生物学作用的发育生物学研究应该受益于这些来自单一和完整物种的快速MALDI-MS谱。费雷拉角R.，S. a.萨赖瓦河R.卡塔里诺，J.S.加西亚角，澳-地C.戈佐湾B。桑维多湖F. a.桑托斯，E. G. Lo Turco，J. H. F. Pontes，A. C.巴索河贝尔托拉河Sartori，M. M. Guardians，F. Perecin，F. V. Meirelles，J. R. Sangalli和M. n.艾柏林单胚胎和卵母细胞的质谱脂质指纹图谱。J. Lipid Res. 2010. 51：1218 - 1227。
Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid ( represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species.-Ferreira, C. R., S. A. Saraiva, R. R. Catharino, J. S. Garcia, F. C. Gozzo, G. B. Sanvido, L. F. A. Santos, E. G. Lo Turco, J. H. F. Pontes, A. C. Basso, R. P. Bertolla, R. Sartori, M. M. Guardieiro, F. Perecin, F. V. Meirelles, J. R. Sangalli, and M. N. Eberlin. Single embryo and oocyte lipid fingerprinting by mass spectrometry. J. Lipid Res. 2010. 51: 1218-1227.