Efferent vagal fibre stimulation blunts nuclear factor-κB activation and protects against hypovolemic hemorrhagic shock (Publication with Expression of Concern. See vol. 146, 2022)
Efferent vagal fibre stimulation blunts nuclear factor-κB activation and protects against hypovolemic hemorrhagic shock (Publication with Expression of Concern. See vol. 146, 2022)
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DOI：
10.1161/01.cir.0000050627.90734.ed
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发表时间：
2003-03-04
期刊：
影响因子：
37.8
通讯作者：
Squadrito, F
中科院分区：
文献类型：
--
作者：
Guarini, S;Altavilla, D;Squadrito, F
背景-我们调查是否传出迷走神经的电刺激（STIM）可以抑制核因子（NF）-κ B激活和出血性（Hem）shock.Methods和Results-Rats炎症级联反应进行双侧颈迷走神经切断术（VGX）或假手术程序。通过间歇性抽血诱导Hem休克，直到平均动脉压稳定在35至40 mm Hg的范围内。恒定电压脉冲在尾侧迷走神经末端的应用（STIM; 5 V，2 ms，1 Hz持续12分钟，平均动脉压稳定后5分钟）延长存活时间（VGX+Hem+假STIM=38+/-3分钟; VGX+Hem+STIM>180分钟），恢复了明显的低血压（VGX+Hem+Sham STIM=33+/-3 mmHg; VGX+Hem+STIM=66+/-5 mmHg），抑制IkappaB α肝丢失，减弱增强的NF-κ B活性，降低肝肿瘤坏死因子（TNF）-α mRNA（VGX+Hem+Sham STIM=1.42+/-0.5量的TNF-α m-RNA; VGX+Hem+STIM=0.51+/-0.2量的TNF-α mRNA），和降低的血浆TNF-α（VGX+Hem+Sham STIM=190+/-24 pg/mL; VGX+Hem+STIM=87+/-15 pg/mL）。Chlorisondamine，烟碱受体拮抗剂，减弱迷走神经stimulation. Conclusions的影响，我们的研究结果表明，副交感神经抑制NF-κ B的大脑反对NF-κ B激活在肝脏和急性低血容量失血性休克过程中的炎症反应。
Background-We investigated whether electrical stimulation (STIM) of efferent vagus nerves may suppress nuclear factor (NF)-kappaB activation and the inflammatory cascade in hemorrhagic (Hem) shock.Methods and Results-Rats were subjected to bilateral cervical vagotomy (VGX) or sham surgical procedures. Hem shock was induced by intermittent withdrawing of blood until mean arterial pressure stabilized within the range of 35 to 40 mm Hg. Application of constant voltage pulses to the caudal vagus ends (STIM; 5 V, 2 ms, 1 Hz for 12 minutes, 5 minutes after mean arterial pressure stabilization) increased survival time (VGX+Hem+Sham STIM=38+/-3 minutes; VGX+Hem+STIM>180 minutes), reverted the marked hypotension (VGX+Hem+Sham STIM=33+/-3 mm Hg; VGX+Hem+STIM=66+/-5 mm Hg), inhibited IkappaBalpha liver loss, and blunted the augmented NF-kappaB activity, decreased hepatic tumor necrosis factor (TNF)-alpha mRNA (VGX+Hem+Sham STIM=1.42+/-0.5 amount of TNF-alpha m-RNA; VGX+Hem+STIM=0.51+/-0.2 amount of TNF-alpha mRNA), and reduced plasma TNF-alpha (VGX+Hem+Sham STIM=190+/-24 pg/mL; VGX+Hem+STIM=87+/-15 pg/mL). Chlorisondamine, a nicotinic receptor antagonist, abated the effects of vagal stimulation.Conclusions-Our results show a parasympathetic inhibition of NF-kappaB by which the brain opposes NF-kappaB activation in the liver and modulates the inflammatory response during acute hypovolemic hemorrhagic shock.