Comparison of physician- and self-collected genital specimens for detection of human papillomavirus in men
Comparison of physician- and self-collected genital specimens for detection of human papillomavirus in men
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DOI：
10.1128/jcm.44.2.513-517.2006
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发表时间：
2006-02-01
影响因子：
9.4
通讯作者：
Ning, L
中科院分区：
文献类型：
--
作者：
Hernandez, BY;McDuffie, K;Ning, L
目前对于检测男性生殖器人乳头瘤病毒 (HPV) 的最合适采样方法尚未达成共识。我们采用了最近开发的一种采集方法，通过摩擦和湿润擦拭生殖器皮肤表面来检测 136 名夏威夷大学附属男性的 HPV。将医生使用此方法采集的生殖器样本与 24 小时后从同一个体自行采集的样本进行比较。与医生采集的标本相比，自行采集的标本获得的充足标本比例更大。医生采集的样本和自行采集的样本中 HPV 检测结果具有可比性；阴茎干的检出率最高（分别为 51.2% 和 51.5%，P = 0.96），其次是阴囊（41.2% 和 46.2%，P = 0.43）、龟头/冠状沟（31.9% 和 33.1%，P = 0.84）和包皮（33.3% 和 28.6%，P = 0.84）。 = 0.74）。配对的医生和自己采集的样本之间 HPV 检测的位点特异性一致性范围从阴茎干的 67.2% (kappa = 0.34) 到包皮的 95.0% (kappa = 0.89)。 HPV 阳性对中的 HPV 基因型具有高度一致性。最常见的类型是 HPV 84 型，约占样本的 15%。金刚砂纸拭子方法为男性生殖器 HPV DNA 检测提供了一种有效的采样方法，可在临床环境内外使用。
There is currently no consensus regarding the most appropriate methods of sampling for the detection of genital human papillomavirus (HPV) in men. We employed a recently developed collection method involving abrasion and moistened swabbing of the genital skin surface for the detection of HPV in a cohort of 136 university-affiliated males in Hawaii. Genital specimens collected by physicians using this method were compared with self-collected specimens from the same individuals obtained 24 h later. Self-collected specimens yielded a greater proportion of sufficient specimens than physician-collected specimens. HPV detection was comparable in physician- and self-collected specimens; detection was highest in the penile shaft (51.2% and 51.5%, respectively, P = 0.96), followed by the scrotum (41.2% and 46.2%, P = 0.43), the glans/coronal sulcus (31.9% and 33.1%, P = 0.84), and the foreskin (33.3% and 28.6%, P = 0.74). Site-specific agreement in HPV detection between paired physician- and self-collected samples ranged from 67.2% (kappa = 0.34) for the penile shaft to 95.0% (kappa = 0.89) for the foreskin. There was a high degree of concordance in HPV genotypes in HPV-positive pairs. The most common type was HPV type 84, which comprised approximately 15% of the specimens. The emery paper-swab method offers an efficient sampling method for genital HPV DNA detection in men that could be used both within and outside of the clinical setting.