Secretion of androgen binding protein by Sertoli cells is influenced by contact with germ cells.
Secretion of androgen binding protein by Sertoli cells is influenced by contact with germ cells.
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支持细胞分泌雄激素结合蛋白受到与生殖细胞接触的影响。
DOI：
10.1002/j.1939-4640.1984.tb00806.x
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发表时间：
1984
影响因子：
--
通讯作者：
M. Stefanini
中科院分区：
文献类型：
--
作者：
M. Galdieri;L. Monaco;M. Stefanini
将支持细胞单独培养或与生殖细胞一起培养，以评价与生殖细胞联合对支持细胞分泌活性的影响。雄激素结合蛋白，这是专门分泌的支持细胞，分泌测定在几个实验条件下。使用以下实验模型：1）来自青春期前动物的生精上皮外植体的培养物，其中存在粘附于支持细胞的生殖细胞（支持细胞富集培养物）; 2）通过从支持细胞富集培养物中去除生殖细胞获得的仅由支持细胞形成的单层，以及3）仅支持细胞培养物和处于确定分化阶段的生殖细胞群的共培养物。所得结果表明，与富集的支持细胞培养物相比，仅支持细胞培养物中FSH诱导的ABP分泌大大降低，并且这种差异在培养的前8天中保持稳定。此外，当共培养的生殖细胞处于分化阶段时，仅支持细胞培养物与生殖细胞的共培养物诱导ABP增加，例如粗线期精母细胞，其能够识别并牢固地粘附于支持细胞单层。与圆形精子细胞共培养，不坚持支持细胞，没有增加FSH诱导ABP生产量。添加淋巴细胞和成纤维细胞等非胚细胞也不能有效刺激ABP分泌。支持细胞和共培养的生殖细胞之间的表面相互作用似乎是必要的FSH诱导ABP生产。（250字处删节）
Sertoli cells were cultured alone or with germ cells to evaluate the effect of the association with germ cells on the secretory activity of Sertoli cells. Secretion of androgen-binding protein, which is specifically secreted by Sertoli cells, was measured under several experimental conditions. The following experimental models were utilized: 1) cultures of explants of seminiferous epithelium from prepubertal animals in which germ cells adherent to Sertoli cells are present (Sertoli cell enriched cultures); 2) monolayers formed only by Sertoli cells, obtained by removing germ cells from Sertoli cell enriched cultures, and 3) cocultures of Sertoli cell only cultures and germ cell populations at defined stages of differentiation. The results obtained indicated that FSH-induced ABP secretion was greatly reduced in Sertoli cell only cultures as compared to enriched Sertoli cell cultures, and that this difference was stable throughout the first eight days of culture. In addition, cocultures of Sertoli cell only cultures with germ cells induced an increase of ABP when cocultured germ cells were at differentiation stages, such as pachytene spermatocytes, which are able to recognize and firmly adhere to the Sertoli cell monolayers. Cocultures with round spermatids, which do not adhere to Sertoli cells, did not increase the amount of FSH-induced ABP production. The addition of nongerminal cells such as lymphocytes and fibroblasts were also not effective in stimulating ABP secretion. Surface interaction between Sertoli cells and cocultured germ cells seemed to be necessary for this FSH-induced ABP production.(ABSTRACT TRUNCATED AT 250 WORDS)