Distinct 1- and 2-monoacylglycerol kinase activities of diacylglycerol kinase isozymes.
Distinct 1- and 2-monoacylglycerol kinase activities of diacylglycerol kinase isozymes.
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二酰基甘油激酶同工酶具有独特的 1- 和 2- 单酰基甘油激酶活性。
DOI：
10.1016/j.bbapap.2016.06.012
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
F.
中科院分区：
文献类型：
--
作者：
Sato;Y.;Murakami;C.;Yamaki;A.;Mizuno;S.;Sakai;H. and Sakane;F.
甘油二酯激酶（DGK）由10种同工酶组成，参与多种病理生理过程。然而，DGKs的酶学性质尚未完全了解。本研究对10种DGK同工酶的1-单酰基甘油激酶（MGK）和2-MGK活性进行了综合分析。我们发现，I型（α、β和γ）、II型（δ、η和κ）和III型（ε）DGKs具有与它们的DGK活性相比的7.9-19.2%的2-MGK活性，而它们的1-MGK活性<3.0%。IV型DGKs（α和β）的1-MGK和2-MGK活性相对于其DGK活性均< 1%。有趣的是，与其DGK活性相比，V型DGKθ具有约6%的1-MGK活性和< 2%的2-MGK活性。纯化的DGKθ也显示出相同的结果，表明其1-MGK活性是内在的。因此，DGK同工酶根据其1-MGK和2-MGK活性分为三种类型：（1）2-MGK活性相对强于其1-MGK活性的那些（I-III型），（2）仅具有可忽略的1-MGK和2-MGK活性的那些（IV型），和（3）1-MGK活性强于其2-MGK活性的那些（V型）。DGKθ的1-MGK活性和DGKα的2-MGK活性均强于目前报道的1-MGK和2-MGK活性。1-MGK和2-MGK活性的存在或不存在可能是每种DGK同工酶的病理生理功能所必需的。
Diacylglycerol kinase (DGK) consists of ten isozymes and is involved in a wide variety of patho-physiological events. However, the enzymological properties of DGKs have not been fully understood. In this study, we performed a comprehensive analysis on the 1-monoacylglycerol kinase (MGK) and 2-MGK activities of ten DGK isozymes. We revealed that type I (α, β and γ), type II (δ, η and κ) and type III (ε) DGKs have 7.9–19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities were < 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (ζ and ι) were < 1% relative to their DGK activities. Intriguingly, type V DGKθ has approximately 6% 1-MGK activity and < 2% 2-MGK activity compared to its DGK activity. Purified DGKθ exhibited the same results, indicating that its 1-MGK activity is intrinsic. Therefore, DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I–III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKθ and the 2-MGK activity of DGKα were stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date. The presence or absence of 1-MGK and 2-MGK activities may be essential to the patho-physiological functions of each DGK isozyme.