Dcr-1 maintains Drosophila ovarian stem cells
Dcr-1 maintains Drosophila ovarian stem cells
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DOI：
10.1016/j.cub.2007.01.050
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发表时间：
2007-03-20
期刊：
影响因子：
9.2
通讯作者：
Xie, Ting
中科院分区：
文献类型：
--
作者：
Jin, Zhigang;Xie, Ting
MicroRNAs(MiRNAs)通过控制特定mRNAs的周转和/或翻译来调节基因的表达。在果蝇中，DICER-1(DCR-1)对于从相应的前体生成成熟的miRNAs是必不可少的。由于已知miRNAs调节发育事件，如许多物种中细胞命运的决定和维持，我们研究了DCR-1的缺乏是否会通过专门从干细胞中移除干细胞(生殖系干细胞，GSCs；体细胞干细胞，SSCs)来影响果蝇卵巢干细胞的维持。我们的结果表明，DCR-1突变的GSC不能维持，并且在没有明显的细胞死亡特征的情况下迅速从生态位中消失，这表明DCR-1控制GSC的自我更新而不是存活。然而，大理石袋(Bag of Marble，bam)基因在DCR-1突变的GSCs中不上调，它的去除并不能减缓DCR-1突变的GSC的丢失，这表明DCR-1通过抑制Bam非依赖的分化途径来控制GSC的自我更新。此外，DCR-1对维持果蝇卵巢中的SSCs也是必不可少的。我们的数据表明，DCR-1产生的miRNAs是维持果蝇卵巢中两种类型的STAR细胞所必需的。
MicroRNAs (miRNAs) regulate gene expression by controlling the turnover, translation, or both of specific mRNAs. In Drosophila, Dicer-1 (Dcr-1) is essential for generating mature miRNAs from their corresponding precursors. Because miRNAs are known to modulate developmental events, such as cell fate determination and maintenance in many species, we investigated whether a lack of Dcr-1 would affect the maintenance of stem cells (germline stem cells, GSCs; somatic stem cells, SSCs) in the Drosophila ovary by specifically removing its function from the stem cells. Our results show that dcr-1 mutant GSCs cannot be maintained and are lost rapidly from the niche without discernable features of cell death, indicating that Dcr-1 controls GSC self-renewal but not survival. bag of marbles (bam), the gene that encodes an important differentiating factor in the Drosophila germline, however, is not upregulated in dcr-1 mutant GSCs, and its removal does not slow down dcr-1 mutant GSC loss, suggesting that Dcr-1 controls GSC self-renewal by repressing a Bam-independent differentiation pathway. Furthermore, Dcr-1 is also essential for the maintenance of SSCs in the Drosophila ovary. Our data suggest that miRNAs produced by Dcr-1 are required for maintaining two types of stern cells in the Drosophila ovary.