Immunophenotyping of the Medullary B Cell Compartment In Mouse Models
Immunophenotyping of the Medullary B Cell Compartment In Mouse Models
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DOI：
10.1007/978-1-0716-1425-9_8
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发表时间：
2021-01-01
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影响因子：
--
通讯作者：
Espeli, Marion
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文献类型：
--
作者：
Bonaud, Amelie;Balabanian, Karl;Espeli, Marion
B细胞的发育是一个循序渐进的过程，包括几个可以在表型上区分的B细胞前体阶段，这发生在成年人的骨髓中。有趣的是，在骨髓中，B细胞前体与这一谱系中分化程度最高的行为者--浆细胞共存。在这一章中，我们描述了一种从骨髓中恢复细胞的方法和一种流式细胞术方法来识别存在于骨髓室中的B细胞谱系的每个亚群。该方案侧重于膜标记来区分所有的B细胞亚群，以便在实验和进一步分析期间保持细胞的完整性。
B cell development is a stepwise process encompassing several B cell precursor stages that can be phenotypically distinguished, and that is taking place in the bone marrow in adults. Interestingly, within the bone marrow B cell precursors coexist with the most differentiated actors of this lineage, the plasma cells. In this chapter, we describe a method to recover cells from the bone marrow and a flow cytometric approach to identify each subpopulation of the B cell lineage that resides within the bone marrow compartment. This protocol focuses on membrane markers to discriminate all the B cell subpopulations in order to preserve cell integrity during experimentation and for further analyses.