Non-glucan attached proteins of Candida albicans biofilm formed on various surfaces.
Non-glucan attached proteins of Candida albicans biofilm formed on various surfaces.
复制标题
白色念珠菌生物膜的非葡聚糖附着蛋白在各种表面上形成。
DOI：
10.1007/s11046-005-0167-2
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发表时间：
2006
期刊：
影响因子：
5.5
通讯作者：
Chaffin,WLajean
中科院分区：
文献类型：
--
作者：
Vediyappan,Govindsamy;Chaffin,WLajean
检测了两种导管表面和义齿丙烯酸树脂表面形成的白念珠菌生物膜的细胞表面和细胞外基质的非葡聚糖附着蛋白。这些蛋白质的SDS-PAGE蛋白质图谱与从嗜热酵母细胞和芽管获得的蛋白质图谱相比大体相似。这一观察结果表明，这类生物膜表面蛋白不是由一组独特的细胞外蛋白组成，或者一种或几种蛋白质主导生物膜的非葡聚糖附着蛋白。然而，在从在一个导管表面上形成的生物膜获得的蛋白质和两种蛋白质Grp 2 p和ORF 19.822p中观察到差异，这两种蛋白质在二维分离后通过质谱法鉴定。这些蛋白质以前与耐药性有关，它们的存在或丰度似乎受到生物膜形成表面的影响。
Non-glucan attached proteins of the cell surface and extracellular matrix ofCandida albicansbiofilms formed on two catheter surfaces and denture acrylic were examined. The SDS-PAGE protein profiles of these proteins compared with that obtained from planktonic yeast cells and germ tubes were generally similar. This observation suggested that this class of biofilm surface proteins is not composed of a unique set of extracellular proteins or that one or a few proteins dominate the non-glucan attached proteins of biofilm. However, differences were observed in the proteins obtained from biofilm formed on one catheter surface and two proteins, Grp2p and ORF19.822p, identified by mass spectrometry following two-dimensional separation. These proteins have previously been associated with drug resistance and their presence or abundance appeared to be influenced by the surface on which the biofilm was formed.