CHITIN OLIGOSACCHARIDES ELICIT LIGNIFICATION IN WOUNDED WHEAT LEAVES
CHITIN OLIGOSACCHARIDES ELICIT LIGNIFICATION IN WOUNDED WHEAT LEAVES
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DOI：
10.1016/0885-5765(89)90012-x
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发表时间：
1989-01-01
影响因子：
2.7
通讯作者：
RIDE, JP
中科院分区：
文献类型：
--
作者：
BARBER, MS;BERTRAM, RE;RIDE, JP
从甲壳素和壳聚糖的酸解产物中分别提纯了甲壳素的低聚体(单体到六聚体)及其脱乙酰化的衍生物(单体到四聚体)，并测定了损伤小麦叶片的木质化诱导活性。在每个伤口40微克时，甲壳素单体和二聚体无效，而甲壳素三聚体具有非常轻微的活性。相反，甲壳素四聚体、五聚体和六聚体均具有显著的激发子活性。剂量-反应曲线表明，激发子活性的甲壳素低聚体的活性相似，并且它们的活性在较低剂量时迅速降低。用几丁质酶消化四聚体后，激发子活性消失。被测试的去乙酰化低聚物(从单体到四聚体)都没有任何显著的激发子活性，证实了乙酰基对激发子活性的要求。甲壳素四聚体的活性完全依赖于检测中包含的损伤前步骤，这解释了为什么以前的研究未能识别出激发子活性的寡聚体。先前的伤害也增加了其他一些已知的木质化激发子的活性。
Oligomers of chitin (monomer to hexamer) and their deacetylated derivatives (monomer to tetramer) were purified from acid hydrolysates of chitin and chitosan, respectively, and assayed for lignification-eliciting activity in wounded wheat leaves. AT 40 .mu.g per wound, the chitin monomer and dimer were inactive, whilst the chitin trimer possessed very slight activity. In contrast, the chitin tetramer, pentamer and hexamer all possessed significant elicitor activity. Dose-response curves indicated that the activities of the elicitor-active chitin oligomers were similar, and that their activity rapidly diminished at lower doses. Digestion of the tetramer with chitinase abolished elicitor activity. None of the deacetylated oligomers tested (monomer to tetramer) possessed any significant elicitor activity, confirming the requirement of acetyl groups for elicitor activity. The activity of the chitin tetramer was entirely dependent on the inclusion of a pre-wounding step in the assay, which explains why a previous study failed to identify an elicitor-active oligomer. Prior wounding also increased the activity of a number of other known lignification elicitors.