Substrates of the MAPK Slt2: Shaping Yeast Cell Integrity.
Substrates of the MAPK Slt2: Shaping Yeast Cell Integrity.
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DOI：
10.3390/jof8040368
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发表时间：
2022-04-04
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影响因子：
--
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酿酒酵母芽期细胞壁完整性(CWI)MAPK通路专用于响应细胞壁损伤，但正在进行的研究表明，它参与了许多其他胁迫条件，表明它具有功能多样性。这条途径的输出主要由MAPK SLT2的活性驱动，它调节酵母生理的重要过程，如通过CWI和其他途径微调信号，响应细胞壁损伤而转录激活，细胞周期，或决定某些细胞器的命运。为此，SLT2精确地磷酸化蛋白质底物，调节它们的活性、稳定性、蛋白质相互作用和亚细胞定位。在这里，在总结了发现被SLT2磷酸化的蛋白质的方法之后，我们回顾了这种MAPK的真实底物和仍有待证实的越来越多的候选底物。在MAPK信号调控的复杂性背景下，我们讨论了SLT2如何通过这些底物的磷酸化来决定酵母细胞的完整性。来自大规模分析的越来越多的数据和现有的方法学方法为及早确定新的SLT2底物和功能铺平了道路。
The cell wall integrity (CWI) MAPK pathway of budding yeast Saccharomyces cerevisiae is specialized in responding to cell wall damage, but ongoing research shows that it participates in many other stressful conditions, suggesting that it has functional diversity. The output of this pathway is mainly driven by the activity of the MAPK Slt2, which regulates important processes for yeast physiology such as fine-tuning of signaling through the CWI and other pathways, transcriptional activation in response to cell wall damage, cell cycle, or determination of the fate of some organelles. To this end, Slt2 precisely phosphorylates protein substrates, modulating their activity, stability, protein interaction, and subcellular localization. Here, after recapitulating the methods that have been employed in the discovery of proteins phosphorylated by Slt2, we review the bona fide substrates of this MAPK and the growing set of candidates still to be confirmed. In the context of the complexity of MAPK signaling regulation, we discuss how Slt2 determines yeast cell integrity through phosphorylation of these substrates. Increasing data from large-scale analyses and the available methodological approaches pave the road to early identification of new Slt2 substrates and functions.
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影响因子：
3.5
作者：
Ahmadpour, Doryaneh;Maciaszczyk-Dziubinska, Ewa;Hohmann, Stefan
通讯作者：
Hohmann, Stefan
影响因子：
11.8
作者：
Cooper, Katrina F.;Khakhina, Svetlana;Kim, Stephen K.;Strich, Randy
通讯作者：
Strich, Randy
影响因子：
16
作者：
Chang, CI;Xu, BE;Goldsmith, EJ
通讯作者：
Goldsmith, EJ
影响因子：
16.6
作者：
Duch, Alba;Canal, Berta;Posas, Francesc
通讯作者：
Posas, Francesc
影响因子：
11.4
作者：
Cooper, KF;Mallory, MJ;Strich, R
通讯作者：
Strich, R