Tissue-specific Spred-2 promoter activity characterized by a gene trap approach
Tissue-specific Spred-2 promoter activity characterized by a gene trap approach
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DOI：
10.1016/j.modgep.2005.08.003
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发表时间：
2006-03-01
影响因子：
1.2
通讯作者：
Schuh, K
中科院分区：
文献类型：
--
作者：
Bundschu, K;Gattenlöhner, S;Schuh, K
Spreads（Sprouty-related proteins with a Ena/Vasodilator-stimulated phosphoprotein homology-1 domain）是一个新的抑制丝裂原活化蛋白激酶（MAPK）信号通路的蛋白质家族。不同的RNA和蛋白质研究已经揭示了几乎无处不在的Spred-2表达模式。但到目前为止，只有很少的数据可用于原位Spred-2启动子活性。在这里，我们显示了一个详细的原位分析的小鼠品系与一个被困的Spred-2基因，使β-半乳糖苷酶和新霉素融合基因（β-geo）的内源性Spred-2启动子的控制下。这使我们能够监测几乎每个器官及其相应亚区室中的Spred-2启动子活性。新生小鼠和成年小鼠的X-Gal染色显示了几乎一致的Spred-2启动子活性模式。我们详细的数据提供了信息，通过鉴定具有强Spred-2启动子活性的组织，进一步研究Spred-2在各种器官中仍然是谜一样的生理功能。(C)2005 Elsevier B. V.保留所有权利。
Spreds (Sprouty-related proteins with an Ena/Vasodilator-stimulated phosphoprotein homology-1 domain) are a new protein family inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway. Different RNA and protein studies already revealed an almost ubiquitous Spred-2 expression pattern. But until now, only few data were available on the in situ Spred-2 promoter activity. Here, we show a detailed in situ analysis of a mouse strain with a trapped Spred-2 gene, bringing a beta-galactosidase and neomycin fusion gene (beta-geo) under the control of the endogenous Spred-2 promoter. This allowed us to monitor Spred-2 promoter activity in practically every organ and their corresponding sub-compartments. X-Gal staining of newborn and adult mice revealed a nearly congruent Spred-2 promoter activity pattern. Our detailed data provide information for further studies of the still enigmatic physiological functions of Spred-2 in various organs by identifying the tissues with strong Spred-2 promoter activity. (C) 2005 Elsevier B.V. All rights reserved.