Genomic characterization of the mouse inhibitor of apoptosis protein 1 and 2 genes
Genomic characterization of the mouse inhibitor of apoptosis protein 1 and 2 genes
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DOI：
10.1006/geno.1997.5059
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发表时间：
1997-12-15
期刊：
影响因子：
4.4
通讯作者：
Korneluk, RG
中科院分区：
文献类型：
--
作者：
Liston, P;Lefebvre, C;Korneluk, RG
分离和鉴定了小鼠凋亡抑制蛋白1和2(Miap1和Miap2)的基因组和cDNA克隆。编码602个氨基酸的MIAP1蛋白和612个氨基酸的MIAP2蛋白的基因以头尾串联的方式包含在一个57kb的基因座中。Miap1基因由9个外显子组成，跨度为24kb，Miap2基因由7个外显子组成，跨度为21kb。荧光原位杂交分析将该基因定位在9A2染色体上，该染色体与包含人类同源基因HIAP1和HIAP2的人类11q22-q23区域的部分区域同源。对完整的Miap1和Miap2 cDNAs进行测序发现，Miap2转录本中有一个异常长的5‘非翻译区，这可能表明非扫描核糖体启动了翻译。(C)1997年学术出版社。
Genomic and cDNA clones encoding mouse inhibitor of apoptosis protein 1 and 2 (Miap1 and Miap2) were isolated and characterized. The genes encoding the 602-amino-acid MIAP1 protein and the 612-amino-acid MIAP2 protein are contained within a 57-kb locus in a tandem head-to-tail arrangement. The Miap1 gene consists of nine exons spanning 24 kb, and the Miap2 gene consists of seven exons spanning 21 kb. Fluorescence in situ hybridization analysis mapped the locus to chromosome 9A2, which is syntenic with portions of the human 11q22-q23 region containing the human homologues HIAP1 and HIAP2. Sequencing of the complete Miap1 and Miap2 cDNAs revealed an unusually long 5' untranslated region in the Miap2 transcript, which may indicate nonscanning ribosomal initiation of translation. (C) 1997 Academic Press.