Persistence of Escherichia coli O157:H7, Salmonella Newport, and Salmonella Poona in the gut of a free-living nematode, Caenorhabditis elegans, and transmission to progeny and uninfected nematodes
Persistence of Escherichia coli O157:H7, Salmonella Newport, and Salmonella Poona in the gut of a free-living nematode, Caenorhabditis elegans, and transmission to progeny and uninfected nematodes
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DOI：
10.1016/j.ijfoodmicro.2004.11.043
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发表时间：
2005-05-25
影响因子：
5.4
通讯作者：
Beuchat, LR
中科院分区：
文献类型：
--
作者：
Kenney, SJ;Anderson, GL;Beuchat, LR
研究了大肠埃希菌O157：H7和沙门氏菌在自由生活线虫肠道中的存留情况。研究温度和相对湿度对秀丽隐杆线虫的影响，并确定受感染的蠕虫是否将Newport型肠道沙门氏菌传播给后代和未感染的蠕虫。给蠕虫喂食非致病性大肠杆菌(OP50)、O157：H7、肠杆菌血清型Newport和肠杆菌串珠型Poona的细胞，然后在4、20或37℃下孵育最多5天。在K琼脂上，摄取病原菌的初始数量在20℃下的1天内显著增加2.93log(10)cfu/虫，并在随后的4天内保持不变。将虫子放置在BACTO琼脂上，摄取的病原菌数量在4T时保持不变，在20T时显著下降，在37℃时在3天内显著增加。Wortris喂食E.coliOP50或S.Newport，在4℃或20℃，相对湿度33%下培养。75%，或98%，以确定摄入细菌的生存特征。与较高的相对湿度相比，在33%的相对湿度下孵育的病原体细胞较少。在20摄氏度和33%相对湿度下孵育的蠕虫中，摄入的大肠杆菌OP50和S.Newport的数量分别下降到1.65和3.44log(10)cfu/虫。在咽部绿色荧光蛋白(GFP)标记的成虫和未感染的野生型蠕虫的K琼脂上放置在一起，可使病原体转移到野生型蠕虫的肠道。S.Newport是从两代不接触这种病原体的线虫中分离出来的。这些研究的结果表明，线虫可能是食源性病原体的临时储存库，也可能是污染收获前水果和蔬菜的媒介，从而潜在地增加与食用生农产品相关的肠道感染的风险。(C)2005 Elsevier B.V.保留所有权利。
A study was undertaken to determine the persistence of Escherichia coli O157:H7 and salmonellae in the gut of a free-living nematode. Caenorhabditis elegans, as affected by temperature and relative humidity and to determine if infected worms transmit Salmonella enterica serotype Newport to progeny and uninfected worms. Worms were fed cells of a non-pathogenic strain of E. coli (OP50), E. coli O157:H7, S. enterica serotype Newport, and S. enterica scrotype Poona, followed by incubating at 4, 20, or 37 degrees C for up to 5 days. initial populations of ingested pathogens significantly increased by up to 2.93 log(10) cfu/worm within 1 day at 20 degrees C on K agar and remained constant for an additional 4 days. When worms were placed on Bacto agar, Populations of ingested pathogens remained constant at 4 T, decreased significantly at 20 T, and increased significantly at 37 degrees C within 3 days. Wortris fed E. coli OP50 or S. Newport were incubated at 4 or 20 degrees C at relative humidities of 33%. 75%, or 98% to determine survival characteristics of ingested bacteria. Fewer cells of the pathogens survived incubation at 33% relative humidity compared to higher relative humidities. Populations of ingested E. coli OP50 and S. Newport decreased by LIP to 1.65 and 3.44 log(10) cfu/worm, respectively, in worms incubated at 20 degrees C and 33% relative humidity. Placement together on K agar of adult worms, labeled with green fluorescent protein (gfp) in the pharynx area, that had ingested gfP-labeled S. Newport and uninfected wild type worms resulted in transfer of the pathogen to gut of wild type worms. S. Newport was isolated from C elegans two generations removed from exposure to the pathogen. Results of these studies show that C. elegans may serve as a temporary reservoir of foodborne pathogens, and could perhaps, be a vector for contaminating preharvest fruits and vegetables, thus potentially increasing the risk of enteric infections associated with consumption of raw produce. (c) 2005 Elsevier B.V. All rights reserved.