Gut bacterial profile in patients newly diagnosed with treatment-naive Crohn's disease
Gut bacterial profile in patients newly diagnosed with treatment-naive Crohn's disease
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DOI：
10.2147/ceg.s33858
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发表时间：
2012-01-01
影响因子：
2.4
通讯作者：
Tonjum, Tone
中科院分区：
文献类型：
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作者：
Ricanek, Petr;Lothe, Sheba M.;Tonjum, Tone
目的：本研究的目的是定义挪威患者的肠道细菌植物群的组成与早期克罗恩病（CD）。方法：通过使用非选择性宏基因组学方法，一般的细菌组成，从回肠和结肠粘膜活检的5名受试者，4名患者不同的CD表型，和一个非炎症性肠病控制，其特征在于。结果：克隆文库中共有106个细菌操作分类单位（OTUs），具有丰富的生物多样性。几乎所有的OTU都属于拟杆菌门（CD中42%，对照中71%）或厚壁菌门（CD中42%，对照中28%），除了属于变形菌门的一些OTU（CD中15%，对照中0%）和一些不能归属于门的OTU结论：基于挪威炎症性肠病（IBD）的高发病率，与其他国家的先前发现相比，该初步研究代表了挪威患者肠道微生物群的相关测定。发现挪威CD患者的细菌谱与其他国家CD患者的细菌谱相似。这些发现并不支持特定的细菌组成是某些国家IBD高发病率的主要致病因素。
Objectives: The aim of this study was to define the composition of the gut bacterial flora in Norwegian patients with early stage Crohn's disease (CD).Methods: By using a nonselective metagenomics approach, the general bacterial composition in mucosal biopsies from the ileum and the colon of five subjects, four patients with different phenotypes of CD, and one noninflammatory bowel disease control, was characterized. After partial 16S ribosomal RNA (rRNA) gene sequencing, BLAST homology searches for species identification and phylogenetic analysis were performed.Results: An overall biodiversity of 106 different bacterial operational taxonomic units (OTUs) was detected in the cloned libraries. Nearly all OTUs belonged to the phylae Bacteroidetes (42% in CD, 71% in the control) or Firmicutes (42% in CD, 28% in the control), except for some OTUs that belonged to the phylum Proteobacteria (15% in CD, 0% in the control) and a few OTUs that could not be assigned to a phylum (2% in CD, 1% in the control).Conclusion: Based on the high incidence of inflammatory bowel disease (IBD) in Norway, this pilot study represents a relevant determination of the gut microbiota in Norwegian patients compared to previous findings in other countries. The bacterial profile of Norwegian CD patients was found to be similar to that of CD patients in other countries. The findings do not support a particular bacterial composition as a predominant causative factor for the high incidence of IBD that exists in some countries.