The Role of Actinin-4 in Bladder Cancer Invasion
The Role of Actinin-4 in Bladder Cancer Invasion
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DOI：
10.1016/j.urology.2009.09.037
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发表时间：
2010-02-01
期刊：
影响因子：
2.1
通讯作者：
Kanayama, Hiroomi
中科院分区：
文献类型：
--
作者：
Koizumi, Takahiro;Nakatsuji, Hiroyoshi;Kanayama, Hiroomi
检测膀胱癌中辅肌动蛋白-4的表达水平，特别是其在细胞生长和侵袭过程中的水平。Actinin-4是一种与细胞运动和肿瘤转移相关的肌动蛋白结合蛋白。方法采用实时定量PCR和Western blot方法检测正常膀胱和膀胱癌细胞系中Actinin-4 mRNA和蛋白的相对表达。肌动蛋白-4表达也定位于膀胱癌细胞和组织中使用免疫组化。膀胱癌细胞的生长和侵袭活性进行了评价，使用细胞生长和体外细胞侵袭试验，并与肌动蛋白-4小干扰核糖核酸处理的膀胱癌细胞进行比较。结果肌动蛋白-4 mRNA和蛋白水平升高，膀胱癌细胞是已知的，表现出增加的生长和侵袭活性。蛋白质表达主要见于浸润性膀胱癌细胞和组织的胞浆中。治疗膀胱癌细胞系与辅肌动蛋白-4小干扰核糖核酸抑制的细胞的侵袭潜力，但并没有改变他们的growth.CONCLUSIONS目前的研究表明，辅肌动蛋白-4 mRNA和蛋白水平升高膀胱癌细胞系表现出增加的生长和侵袭活动。此外，actinin-4敲低抑制膀胱癌细胞的侵袭，但不改变其生长。总之，我们推测辅肌动蛋白-4在细胞质中的积累与肿瘤侵袭和转移的易感性增加有关。泌尿学75：357-364，2010年。(C)2010年爱思唯尔公司
OBJECTIVES To examine actinin-4 expression levels in bladder cancer, in particular its levels during cellular growth and invasion. Actinin-4 is an actin-binding protein that is associated with cell motility and cancer metastasis.METHODS Relative messenger ribonucleic acid (mRNA) and protein expression of actinin-4 in normal bladder and bladder cancer cell lines was determined by quantitative real-time polymerase chain reaction and Western blot analysis. Actinin-4 expression was also localized in bladder cancer cells and tissues using immunohistochemistry. The growth and invasion activity of bladder cancer cells was evaluated using cell growth and in vitro cell invasion assays, and compared with that of bladder cancer cells treated with actinin-4 small interfering ribonucleic acids.RESULTS Actinin-4 mRNA and protein levels were elevated in bladder cancer cells that are known to exhibit increased growth and invasion activity. Protein expression was predominantly observed in the cytoplasm of the invasive bladder cancer cells and tissues. Treatment of bladder cancer cell lines with actinin-4 small interfering ribonucleic acids suppressed the invasive potential of the cells, but did not alter their growth.CONCLUSIONS The current study demonstrates that actinin-4 mRNA and protein levels are elevated in bladder cancer cells lines that exhibit increased growth and invasion activity. In addition, actinin-4 knockdown inhibited invasion of bladder cancer cells, but did not alter their growth. In conclusion, we hypothesize that the accumulation of actinin-4 in the cell cytoplasm is related to an increased susceptibility of tumor invasion and metastasis. UROLOGY 75: 357-364, 2010. (C) 2010 Elsevier Inc.