Unveiling the characteristics of popcorn by genome re-sequencing and integrating the ESTs and proteome data
Unveiling the characteristics of popcorn by genome re-sequencing and integrating the ESTs and proteome data
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DOI：
10.1007/s42976-022-00308-8
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发表时间：
2022-12
影响因子：
1.6
通讯作者：
Yongbin Dong;Fei Deng;Long Zhang;Xinyu Li;Qilei Wang;Yu-ling Li
中科院分区：
文献类型：
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作者：
Yongbin Dong;Fei Deng;Long Zhang;Xinyu Li;Qilei Wang;Yu-ling Li
爆米花（Zea mays everta Sturt.）在加热时可以爆炸爆米花片作为零食。本研究以小粒重的玉米自交系N04和大粒重的玉米丹232为材料，分别与B73参考基因组进行了重测序和比较。获得了N04和Dan232之间的基因组变异信息，包括95万个SNPS、44万个INS和49万个DEL。结合墨西哥地方玉米品种Palomero与凹陷玉米自交系Zheng58、Chang7-2和Mo17的基因组，鉴定出10837个玉米与凹陷玉米的序列变异基因。在爆米花基因组中发现308个独特基因，主要参与代谢过程、磷代谢、蛋白质修饰等生物过程。通过分析N04和Dan232籽粒发育的蛋白质组学和基因组数据，共获得318个差异基因。同时在est、基因组和蛋白质组数据中仅发现4个差异表达基因，分别编码生长素结合蛋白、淀粉酶、丙酮酸磷酸激酶和葡萄糖磷酸腺苷转移酶。这些结果揭示了爆米花的基因组特征，为研究爆米花特性的分子机制提供了重要信息。
Popcorn (Zea mays everta Sturt.) can explode popcorn flake for snack food when heated. In this study, a popcorn inbred line, N04 with small grain weight, and a dent corn Dan232 with large grain weight were re-sequenced and compared with the B73 reference genome, respectively. The genome variation information between N04 and Dan232 was obtained, including 0.95 million SNPS, 0.44 million INS and 0.49 million DEL. Combining the genome of Mexican landrace Palomero and dent maize inbred lines Zheng58, Chang7-2 and Mo17, 10,837 genes with sequence variation were identified between popcorn and dent corn. There were 308 unique genes found in the popcorn genome, which were mainly involved in metabolic processes, phosphorus metabolism, protein modification and other biological processes. A total of 318 differential genes were obtained by analyzing proteome and genomic data of kernel development of N04 and Dan232. And only 4 differential expression genes were found among ESTs, genome and proteome data simultaneously, which encode auxin-binding protein, amylase, pyruvate phosphate kinase and glucose phosphate adenylyltransferase, respectively. These results uncover the genome characteristics of popcorn, which will provide important information for studying the molecular mechanism of popping characteristics.