Nanoparticles from photocopiers induce oxidative stress and upper respiratory tract inflammation in healthy volunteers
Nanoparticles from photocopiers induce oxidative stress and upper respiratory tract inflammation in healthy volunteers
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DOI：
10.3109/17435390.2012.691998
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发表时间：
2013-08-01
期刊：
影响因子：
5
通讯作者：
Woskie, Susan
中科院分区：
文献类型：
--
作者：
Khatri, Madhu;Bello, Dhimiter;Woskie, Susan
复印机排放大量纳米颗粒 (NP)；然而，它们的毒理学特性尚未得到研究。在这里，我们首次研究了人类在接触复印机中的纳米粒子一天后的早期反应。九名健康受试者在随机选择的 2-3 天在繁忙的复印中心度过了 6 小时。在暴露前和暴露后不同时间点收集匹配的鼻灌洗液和尿液样本。分析鼻灌洗样本中的 14 种细胞因子、炎症细胞和总蛋白。分析尿液样本中的 8-羟基脱氧鸟苷 (8-OH-dG)。使用一套仪器进行暴露评估。暴露天数的平均总颗粒数比背景高 5 倍以上，尺寸分布在纳米级范围内（峰值 30-40 nm）。暴露后，8-OH-dG 和几种促炎细胞因子比暴露前水平升高 2-10 倍，并在长达 36 小时内保持升高状态。我们得出的结论是，复印机中的纳米颗粒会诱发上呼吸道炎症和氧化应激。
Photocopiers emit large quantities of nanoparticles (NPs); however, their toxicological properties have not been studied. Here we investigate for the first time early human responses following a day's exposure to NPs from photocopiers. Nine healthy subjects spent 6 h at a busy photocopy centre on 2-3 randomly selected days. Matched nasal lavage and urine samples were collected before and at different time points post-exposure. Nasal lavage samples were analysed for 14 cytokines, inflammatory cells and total protein. Urine samples were analysed for 8-hydroxydeoxyguanosine (8-OH-dG). Exposure assessment was conducted using a suite of instruments. The mean total particle number on exposure days was >5 times higher than background, with size distributions in nanoscale range (peak 30-40 nm). Following exposure, 8-OH-dG and several pro-inflammatory cytokines were elevated 2-10 folds compared with pre-exposure levels and remained elevated for up to 36 h. We conclude that NPs from photocopiers induce upper airway inflammation and oxidative stress.