Aging enhances classical activation but mitigates alternative activation in the central nervous system.
Aging enhances classical activation but mitigates alternative activation in the central nervous system.
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DOI：
10.1016/j.neurobiolaging.2012.12.014
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发表时间：
2013-06
影响因子：
4.2
通讯作者：
Morgan D
中科院分区：
文献类型：
--
作者：
Lee DC;Ruiz CR;Lebson L;Selenica ML;Rizer J;Hunt JB Jr;Rojiani R;Reid P;Kammath S;Nash K;Dickey CA;Gordon M;Morgan D
小胶质细胞/巨噬细胞在神经炎症和神经退行性疾病中的作用仍然存在争议。迄今为止，至少有两种激活状态被提出，包括经典响应（M1）和替代响应（M2）。鉴定代表小胶质细胞功能激活状态的选择性生物标志物可能有助于阐明疾病进程和了解修复机制。将含有TNF-α、IL-12和IL-1β（称为CKT-1）或IL-13和IL-4（称为CKT-2）的两种鸡尾酒注射到6、12或24个月大的小鼠海马中。注射后3天对海马组织进行微阵列分析。比较了CKT-1和CKT-2刺激物鸡尾酒之间的基因转录物。CKT-1表达的几个选择性转录本包括CXCL13、haptoglobin、MARCO和calgranulin B，而CKT-2选择性地诱导了一小部分基因，包括FIZZ1、IGF-1和EAR 11。重要的是，CKT-1在所有年龄段都能诱导选择性转录物，而CKT-2诱导的选择性基因转录物随着年龄的增长而减少，这表明IL-4/ IL-13信号通路的年龄相关减少。
The role of microglia/ macrophages during neuroinflammation and neurodegenerative diseases remains controversial. To date, at least two activations states have been suggested consisting of a classical response (M1) and the alternative response (M2). Identifying selective biomarkers of microglia that representative their functional activation states may help elucidate disease course and understand repair mechanisms. Two cocktails containing either TNF-α, IL-12, and IL-1β (referred to as CKT-1) or IL-13 and IL-4 (referred to CKT-2) were injections into the hippocampus of mice aged 6, 12, or 24 months. Microarray analysis was performed on hippocampal tissue 3 days post injection. Gene transcripts were compared between CKT-1 versus CKT-2 stimulator cocktails. Several selective transcripts expressed for the CKT-1 included CXCL13, haptoglobin, MARCO, and calgranulin B, while a smaller subset of genes was selectively induced by the CKT-2 and consisted of FIZZ1, IGF-1, and EAR 11. Importantly, selective transcripts were induced at all ages by CKT-1, whereas selective gene transcripts induced by CKT-2 decreased with age suggesting an age-related reduction in the IL-4/ IL-13 signaling pathway.
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DOI：
10.1523/jneurosci.4403-10.2010
发表时间：
2010-12-15
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Liu Z;Condello C;Schain A;Harb R;Grutzendler J
通讯作者：
Grutzendler J
影响因子：
3.8
作者：
Dörmer, P;Spitzer, E;Möller, W
通讯作者：
Möller, W
影响因子：
4.2
作者：
Maher, FO;Nolan, Y;Lynch, MA
通讯作者：
Lynch, MA
影响因子：
4.8
作者：
Nolan, Y;Maher, FO;Lynch, MA
通讯作者：
Lynch, MA
影响因子：
6.5
作者：
Jung, Min Kyung;Park, Yoorim;Cho, Daeho
通讯作者：
Cho, Daeho