Interleukin-10 down-regulates MHC class II αβ peptide complexes at the plasma membrane of monocytes by affecting arrival and recycling
Interleukin-10 down-regulates MHC class II αβ peptide complexes at the plasma membrane of monocytes by affecting arrival and recycling
复制标题
DOI：
10.1016/s1074-7613(00)80404-5
复制
发表时间：
1997-12-01
期刊：
影响因子：
32.4
通讯作者：
Malefyt, RD
中科院分区：
文献类型：
--
作者：
Koppelman, B;Neefjes, JJ;Malefyt, RD
当将人单核细胞用作抗原呈递细胞时，白介素10（IL-10）抑制抗原特异性T细胞反应。这与单核细胞表面上MHC II类分子的下调有关。在这里，我们表明IL-10不会影响MHC II类转录，多肽合成，亚基组装或抗原肽载荷。取而代之的是，新合成的成熟MHC II类分子位于MHC II类载荷室，但阻止到达质膜。此外，用IL-10的单核细胞处理导致内部MHC II类复合物在细胞内囊泡中的积累。这些结果表明，IL-10通过调节MHC胞吐作用和回收利用会影响抗原表现。
Interleukin-10 (IL-10) inhibits antigen-specific T cell responses when human monocytes are used as antigen-presenting cells. This is correlated with a downregulation of MHC class II molecules on the surface of the monocyte. Here we show that IL-10 does not affect MHC class II transcription, polypeptide synthesis, subunit assembly, or antigenic peptide loading. Instead, newly synthesized mature MHC class II molecules are localized to the MHC class II loading compartment but are prevented from reaching the plasma membrane. In addition, treatment of monocytes with IL-10 leads to an accumulation of internalized MHC class II complexes in intracellular vesicles. These results indicate that IL-10 affects antigen presentation by regulating MHC exocytosis and recycling.