Decomposition of 14C glucose in two soils with different amounts of heavy metal contamination
Decomposition of 14C glucose in two soils with different amounts of heavy metal contamination
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DOI：
10.1016/s0038-0717(01)00108-0
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发表时间：
2001-10-01
影响因子：
9.7
通讯作者：
Joergensen, RG
中科院分区：
文献类型：
--
作者：
Chander, K;Joergensen, RG
一个高含金属的土壤和一个低含金属的土壤提供了C-14标记的葡萄糖在两个速率，一个提供一个恒定的葡萄糖与土壤的比例和其他一个恒定的葡萄糖与生物量的比例。目的是评估这些不同比例对微生物底物利用效率的影响。在添加底物后不久，加入葡萄糖（含或不含N）以研究熏蒸提取方法的提取效率。添加葡萄糖无N导致微生物生物量C比微生物茚三酮反应性N（E-NIN）在底物添加后的头几天内成比例地增加，由于N缺乏。生物量C-到-E-NIN比保持不变，在所有的土壤处理后，葡萄糖添加与N相结合，表明熏蒸提取方法的提取效率不受葡萄糖的影响。较低百分比的葡萄糖加入到微生物生物质中，葡萄糖与生物质的比例增加。呼吸的生物量纳入C-14的比例增加，在所有高金属含量的土壤处理显着高于低金属含量的土壤从第二天的孵化，显着压倒葡萄糖C-生物量C的比例的影响。我们的研究结果清楚地表明，更多的基板被转移到分解代谢的微生物在合成代谢过程中的高金属含量的土壤。(C)2001爱思唯尔科技有限公司版权所有。
A high metal-containing soil and a low metal-containing soil were supplied with C-14-labelled glucose at two rates, one to provide a constant glucose-to-soil ratio and the other a constant glucose-to-biomass ratio. The aim was to assess the effects of these different ratios on the microbial substrate utilisation efficiency, Glucose was added with or without N to investigate the extraction efficiency of the fumigation-extraction method shortly after substrate addition. The addition of glucose without N resulted in a proportionally larger increase in microbial biomass C than in microbial ninhydrin-reactive N (E-NIN) within the first few days after substrate addition, due to N deficiency. The biomass C-to-E-NIN ratio remained constant in all soil treatments after glucose addition in combination with N, indicating that the extraction efficiency of the fumigation-extraction method is not affected by the addition of glucose. Lower percentages of glucose added were incorporated into the microbial biomass with an increasing ratio of glucose-to-biomass. The ratio of respired to biomass incorporated C-14 increased in all high metal-containing soil treatments markedly above that of the low metal-containing soil from day two of the incubation, markedly overriding the effects on the glucose C-to-biomass C ratio. Our results clearly demonstrated that more substrate was diverted by microorganisms into catabolic at the expense of anabolic processes in a high metal-containing soil. (C) 2001 Elsevier Science Ltd. All rights reserved.