Isolation and characterization of murine neural stem/progenitor cells based on Prominin-1 expression
Isolation and characterization of murine neural stem/progenitor cells based on Prominin-1 expression
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DOI：
10.1016/j.expneurol.2007.03.021
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发表时间：
2007-06-01
影响因子：
5.3
通讯作者：
Comi, Giacomo P.
中科院分区：
文献类型：
--
作者：
Corti, Stefania;Nizzardo, Monica;Comi, Giacomo P.
神经干细胞（NSC）分离策略的确定对于理解其生物学和治疗应用的开发具有重要意义。先前已经描述过，可以使用 Prominin 抗体 (CD133) 和荧光激活细胞分选 (FACS) 从中枢神经系统 (CNS) 中分离人神经干细胞和祖细胞 (NSPC)。尽管这种抗原在几种人类和小鼠组织中显示出相同的膜拓扑结构，但由于氨基酸同一性水平较低，人类和小鼠 prominin 之间的关系存在不确定性。在这里，我们表明 prominin 表达也可用于从发育中或成年大脑中识别和分离小鼠 NSPC。 Prominin 与已知的神经干标记物（如 SOX 1-2、Musashi 和 Nestin）共表达。此外，具有多能性和自我更新能力的神经球形成细胞位于prominin阳性部分内。移植实验表明，CD133 阳性细胞在体内产生神经元和神经胶质细胞，并且许多神经元表现出受体组织的适当表型特征。CD133 是小鼠 NSPC 的干细胞抗原，就像它是人类 NSPC 的干细胞抗原，对于研究哺乳动物神经发生和开发 NSPC 移植到人类疾病的小鼠类似物中的临床前测试是有用的。 (c) 2007 Elsevier Inc. 保留所有权利。
The identification of strategies for the isolation of neural stem cells (NSCs) has important implications for the understanding of their biology and the development of therapeutic applications. It has been previously described that human neural stem and progenitor cells (NSPCs) can be isolated from the central nervous system (CNS) using antibodies to prominin (CD133) and fluorescence-activated cell sorting (FACS). Although this antigen displayed an identical membrane topology in several human and murine tissues there was uncertainty as to the relationship between human and mouse prominin because of the low level of amino acid identity.Here we show that prominin expression can be used to identify and isolate also murine NSPCs from the developing or adult brain. Prominin is co-expressed with known neural stem markers like SOX 1-2, Musashi and Nestin. Moreover, neurosphere-forming cells with multipotency and self-renewal capacity reside within the prominin-positive fraction. Transplantation experiments show that CD133-positive cells give rise to neurons and glial cells in vivo, and that many neurons display appropriate phenotypic characteristics of the recipient tissues.The demonstration that CD133 is a stem cell antigen for murine NSPCs as it is for human NSPCs is useful for the investigation of mammal neurogenesis and development of preclinical tests of NSPCs transplantation in mouse analogues of human diseases. (c) 2007 Elsevier Inc. All rights reserved.