Fibroblasts form a body-wide cellular network
Fibroblasts form a body-wide cellular network
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DOI：
10.1007/s00418-004-0667-z
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发表时间：
2004-07-01
影响因子：
2.3
通讯作者：
Taatjes, DJ
中科院分区：
文献类型：
--
作者：
Langevin, HM;Cornbrooks, CJ;Taatjes, DJ
“松散”结缔组织形成一个网络延伸到整个身体，包括皮下和间质结缔组织。疏松结缔组织内成纤维细胞网络的存在可能具有相当大的意义，因为它可能支持尚不清楚的全身细胞信号系统。我们使用组织化学、免疫组织化学、共聚焦扫描激光显微镜（共聚焦显微镜）和电子显微镜的组合来研究小鼠皮下结缔组织中细胞间连接的程度和性质。我们发现成纤维细胞在整个组织中形成网状网。在共聚焦显微镜下，30%的成纤维细胞的过程可以连续地从一个细胞到另一个细胞。连接蛋白43免疫反应性存在于细胞间接触的明显点。电镜显示相邻细胞的突起彼此紧密相连，但未观察到间隙连接。我们的研究结果表明，软组织成纤维细胞形成了一个广泛相互连接的细胞网络，这表明它们可能在整个身体水平上具有重要的、迄今未被怀疑的综合功能。
"Loose" connective tissue forms a network extending throughout the body including subcutaneous and interstitial connective tissues. The existence of a cellular network of fibroblasts within loose connective tissue may have considerable significance as it may support yet unknown body-wide cellular signaling systems. We used a combination of histochemistry, immunohistochemistry, confocal scanning laser microscopy (confocal microscopy), and electron microscopy to investigate the extent and nature of cell-to-cell connections within mouse subcutaneous connective tissue. We found that fibroblasts formed a reticular web throughout the tissue. With confocal microscopy, 30% of fibroblasts' processes could be followed continuously from one cell to another. Connexin 43 immunoreactivity was present at apparent points of cell-to-cell contact. Electron microscopy revealed that processes from adjacent cells were in close apposition to one another, but gap junctions were not observed. Our findings indicate that soft tissue fibroblasts form an extensively interconnected cellular network, suggesting they may have important and so far unsuspected integrative functions at the level of the whole body.