RNA TRANSCRIPTS FOR I-J POLYPEPTIDES ARE APPARENTLY NOT ENCODED BETWEEN THE I-A AND I-E SUBREGIONS OF THE MURINE MAJOR HISTOCOMPATIBILITY COMPLEX
RNA TRANSCRIPTS FOR I-J POLYPEPTIDES ARE APPARENTLY NOT ENCODED BETWEEN THE I-A AND I-E SUBREGIONS OF THE MURINE MAJOR HISTOCOMPATIBILITY COMPLEX
复制标题
DOI：
10.1073/pnas.80.18.5704
复制
发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
HOOD, L
中科院分区：
文献类型：
--
作者：
KRONENBERG, M;STEINMETZ, M;HOOD, L
据报道，小鼠主要组织相容性复合体的I-J亚区编码由抑制性T细胞表达的抗原决定因子。从小鼠精子DNA中获得的Cosmid克隆包含I-A和I-E亚区之间的所有序列，其中I-J已被遗传定位。将这些序列与从几个i - j阳性抑制性t细胞杂交瘤中制备的RNA杂交，没有发现转录物的存在。在被分析的抑制性T细胞中没有检测到这种DNA的重排。显然，I-J多肽不是在主要组织相容性复合体的I-A和I-E亚区之间编码的。讨论了有关I-J基因可能定位和表达的几种假设。
The I-J subregion of the mouse major histocompatibility complex has been reported to encode antigenic determinants expressed by suppressor T cells. Cosmid clones were obtained from mouse sperm DNA that contain all of the sequences between the I-A and I-E subregions, where I-J has been mapped genetically. Hybridization of these sequences to RNA prepared from several I-J-positive suppressor T-cell hybridomas did not reveal the presence of a transcript. No rearrangements in this DNA were detected in the suppressor T cells that were analyzed. Apparently, the I-J polypeptides are not encoded between the I-A and I-E subregions of the major histocompatibility complex. Several hypotheses concerning the possible location and expression of I-J genes are discussed.