3-DIMENSIONAL RECONSTRUCTION FROM RANDOM PROJECTIONS - ORIENTATIONAL ALIGNMENT VIA RADON TRANSFORMS
3-DIMENSIONAL RECONSTRUCTION FROM RANDOM PROJECTIONS - ORIENTATIONAL ALIGNMENT VIA RADON TRANSFORMS
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DOI：
10.1016/0304-3991(94)90003-5
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发表时间：
1994-02-01
期刊：
影响因子：
2.2
通讯作者：
RADERMACHER, M
中科院分区：
文献类型：
--
作者：
RADERMACHER, M
提出了一种面向三维参考点的未知投影方向对齐方法。该技术已被应用于从大肠杆菌中记录的50S核糖体亚基的冷冻水合制剂的图像的三维重建。本文使用的算法将单曝光圆锥重建技术（SECReT）与三维方向搜索相结合。该算法允许通过改进真实投影角度和包含先验未知随机方向的投影来改进用SECReT获得的重建。模型数据表明，即使在信噪比低于1的情况下，该算法也能可靠地工作。
A method for alignment of projections with unknown projecting directions towards a three-dimensional reference has been developed. The technique has been applied to the three-dimensional reconstruction from images of a frozen-hydrated preparation of 50S ribosomal subunits from Escherichia coli recorded in the electron microscope. The algorithm as used here combines the Single Exposure Conical Reconstruction Technique (SECReT) with a three-dimensional orientation search. The algorithm allows for the refinement of a reconstruction obtained with SECReT by refinement of the true projection angles, and by the inclusion of projections with a priori unknown random orientation. With model data it is demonstrated that the algorithm works reliably even for signal-to-noise ratios lower than 1.