Double strand cleavage of genomic DNA at a single site by triple helix formation
Double strand cleavage of genomic DNA at a single site by triple helix formation
复制标题
通过形成三螺旋在单个位点对基因组 DNA 进行双链切割
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
P. Dervan
中科院分区：
文献类型：
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作者：
S. Strobel;H. Moser;P. Dervan
通过限制性核酸内切酶对双螺旋 DNA 进行序列特异性切割对于分子生物学中的许多技术至关重要，包括基因分离、DNA 测序和重组 DNA 操作。随着脉冲场凝胶电泳的出现，现在可以分离大片段的 DNA。然而，天然存在的限制性酶的识别序列在4-8个碱基对的范围内，因此它们的序列特异性可能不足以从大染色体（大小为10^8个碱基对）中分离基因或绘制基因组DNA图谱。
The sequence-specific cleavage of double helical DNA by restriction endonucleases is essential for many techniques in molecular biology, including gene isolation, DNA sequencing, and recombinant DNA manipulations. With the advent of pulsed-field gel electrophoresis, the separation of large segments of DNA is now possible. However, the recognition sequences of naturally occurring restriction enzymes are in the range of 4-8 base pairs, and hence their sequence specificites may be inadequate for isolating genes from large chromosomes (10^8 base pairs in size) or mapping genomic DNA.