miR164c and miR168a regulate seed vigor in rice
miR164c and miR168a regulate seed vigor in rice
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miR164c 和 miR168a 调节水稻种子活力
DOI：
10.1111/jipb.12792
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发表时间：
2020-04-01
影响因子：
11.4
通讯作者：
Jiang, Xiaocheng
中科院分区：
文献类型：
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作者：
Zhou, Yan;Zhou, Shiqi;Jiang, Xiaocheng
microRNAs（miRNAs）是植物许多重要生物学过程中基因表达的关键调控因子。然而，很少有miRNAs被证明可以调节种子活力。在这里，我们进行了微阵列分析，以分析水稻种子中的miRNA表达水平。品种ZR 02。结果显示，人工老化和未处理的对照种子之间的11个miRNAs的表达存在显着差异。其中，osa-miR 164 c在人工老化种子中转录上调，而osa-miR 168 a下调;这通过定量实时PCR分析验证。在相同老化条件下，Osa-miR 164 c过表达和Osa-miR 168 a沉默的转基因水稻种子发芽率均低于野生型种子，而Osa-miR 164 c沉默和Osa-miR 168 a过表达的转基因水稻种子发芽率高于野生型种子。同时，老化条件下种子的细胞膜透性以及osa-miR 164 c靶基因（OsPM 27和OsPSK 5）和osa-miR 168 a靶基因（OsAGO 1和OsPTR 2）的表达水平的变化与osa-miR 164 c和osa-miR 168 a诱导的种子活力的变化一致。因此，miRNAs的遗传操作对于培育具有高活力和延长种子寿命的作物品种具有重要意义。
MicroRNAs (miRNAs) are key regulators of gene expression in many important biological processes of plants. However, few miRNAs have been shown to regulate seed vigor. Here, we conducted microarray assays to analyze miRNA expression levels in seeds of the rice (Oryza sativa L.) cultivar ZR02. Results showed significant differences in the expression of 11 miRNAs between artificially aged and untreated control seeds. Among these, osa-miR164c was transcriptionally upregulated, while osa-miR168a was downregulated in artificially aged seeds; this was verified by quantitative real-time PCR analysis. Under the same aging condition, osa-miR164c overexpression in OE164c transgenic seeds and osa-miR168a silencing in MIM168a transgenic seeds of the rice cultivar Kasalath led to lower germination rates, whereas osa-miR164c silencing in MIM164c and osa-miR168a overexpression in OE168a resulted in higher seed germination rates compared with wild-type seeds. Meanwhile, changes in cytomembrane permeability of seeds and in the expression level of osa-miR164c target genes (OsPM27 and OsPSK5) and osa-miR168a target genes (OsAGO1 and OsPTR2) under aging conditions coincided with changes in seed vigor induced by osa-miR164c and osa-miR168a. Thus, genetic manipulation of miRNAs has important implications in the development of crop cultivars with high vigor and extended life span of seeds.