Human cytomegalovirus DNA: physical maps for restriction endonucleases BglII, hindIII and XbaI.
Human cytomegalovirus DNA: physical maps for restriction endonucleases BglII, hindIII and XbaI.
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DOI：
10.1099/0022-1317-49-1-1
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发表时间：
1980-07
期刊：
影响因子：
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通讯作者：
M. W. Weststrate;J. Geelen;J. van der Noordaa
中科院分区：
文献类型：
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作者：
M. W. Weststrate;J. Geelen;J. van der Noordaa
提出人巨细胞病毒（HCMV）的基因组由两个独特的序列L和S组成，由两组冗余序列限定（P.Sheldrick等，未发表的数据）。在这种排列中，末端序列（TR 1和TR 8）以内部反向形式（IR 1和IR 8）重复，并界定L和S。在DNA的限制性内切酶切割后，发现4个0.5 M和4个0.25 M片段，表明HCMV DNA制剂由4个等摩尔群体组成，仅在L和S组分的相对方向上不同。用限制性内切酶BglII、HindIII和XbaI切割CMV DNA，分别产生32、27和21个片段。这些片段的排列已确定使用分子杂交技术，识别的末端片段和识别的连锁群，通过双消化。在本报告中，给出了限制性内切酶BglII、HindIII和XbaI的物理图谱。
It is proposed that the genome of human cytomegalovirus (HCMV) consists of two unique sequences, L and S, bounded by two sets of redundant sequences (P. Sheldrick et al. unpublished data). In this arrangement the terminal sequences (TR1 and TR8) are repeated in an intenal inverted form (IR1 and IR8) and delimit L and S. After restriction endonuclease cleavage of the DNA, four o.5 M and four 0.25 M fragments are found, indicating that HCMV DNA preparations consist of four equimolar populations differing only in the relative orientation of the L and S components. Cleavage of the CMV DNA with the restriction endonucleases BglII, HindIII and XbaI results in 32, 27 and 21 fragments, respectively. The arrangement of these fragments has been determined using molecular hybridization techniques, identification of terminal fragments and the identification of linkage groups by double-digestion. In this report the physical maps for the restriction endonucleases BglII, HindIII and XbaI are presented.