PmRunt regulated by Pm-miR-183 participates in nacre formation possibly through promoting the expression of collagen VI-like and Nacrein in pearl oyster Pinctada martensii.
PmRunt regulated by Pm-miR-183 participates in nacre formation possibly through promoting the expression of collagen VI-like and Nacrein in pearl oyster Pinctada martensii.
复制标题
Pm-miR-183调控的PmRunt可能通过促进马氏珠母贝中Collagen VI-like和Nacrein的表达参与珍珠层形成
DOI：
10.1371/journal.pone.0178561
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Huang R
中科院分区：
文献类型：
--
作者：
Zheng Z;Du X;Xiong X;Jiao Y;Deng Y;Wang Q;Huang R
Heterodimeric PEBP2/CBFs are key regulators in diverse biological processes, such as haematopoietic stem-cell generation, bone formation and cancers. In this work, we cloned runt-like transcriptional factor (designated asPmRunt) and CBF β (designated asPmCBF) gene, which comprise the heterodimeric transcriptional factor inPinctada martensii.PmRuntwas identified with an open reading frame that encodes 545 amino acids and has typical Runt domain. Phylogenetic analysis results speculated that runt-like transcriptional factors (RDs) in vertebrates and invertebrates are separated into two branches. In molluscs, PmRunt and other RDs are clustered in one of these branches. Direct interaction between PmRunt and PmCBF was evidenced by yeast two-hybrid assay results. Gene repression by RNA interference decreased the expression level ofPmRunt, and subsequent observation of the inner surface of the nacre by scanning electron microscopy demonstrated disordered growth. The luciferase activities of reporters that contain promoter regions ofCollagen VI-like(PmColVI) andPmNacreinwere enhanced byPmRunt. Meanwhile,Pm-miR-183 apparently inhibited the relative luciferase activity of reporters containing the 3′-UTR ofPmRunt. The expression level ofPmRuntwas repressed after Pm-miR-183 was overexpressed in the mantle tissue. Therefore, we proposed thatPmRuntcould be targeted byPm-miR-183and regulate the transcription ofPmColVIandPmNacreinby increasing their transcriptional activity, thereby governing nacre formation.
