Distinct patterns of lipoproteins with apoB defined by presence of apoE or apoC-III in hypercholesterolemia and hypertriglyceridemia.
Distinct patterns of lipoproteins with apoB defined by presence of apoE or apoC-III in hypercholesterolemia and hypertriglyceridemia.
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发表时间：
2001-08
影响因子：
6.5
通讯作者：
Hannia Campos;Dan Perlov;Christina Khoo;Frank M Sacks
中科院分区：
文献类型：
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作者：
Hannia Campos;Dan Perlov;Christina Khoo;Frank M Sacks
极低密度脂蛋白和低密度脂蛋白中载脂蛋白E和载脂蛋白C-Ⅲ浓度与冠心病相关。我们研究了载脂蛋白E和载脂蛋白C-Ⅲ和载脂蛋白B脂蛋白的异常浓度和分布之间的关系，在10个高胆固醇血症和13个高脂血症患者相比，12个正常血脂受试者（平均年龄，45岁）。首先依次使用抗apoE和抗apoC-Ⅲ免疫亲和层析，然后超离心分离16种不同类型的apoB脂蛋白颗粒[轻VLDL、致密VLDL、IDL和LDL，有或无apoC-Ⅲ（E（+）C-III（+）、E（+）C-III（-））或无apoE有或无apoC-Ⅲ（E（-）C-III（+）、E（-）C-III（-））]。高胆固醇血症组VLDL中载脂蛋白C-Ⅲ（E（+）C-Ⅲ（+），E（-）C-Ⅲ（+））颗粒浓度较高胆固醇血症组和血脂正常组增高。这些颗粒是最富含甘油三酯的颗粒类型，与其他两组相比，高血脂症患者的甘油三酯含量是其他两组的两倍。高脂血症组E（-）C-III（-）颗粒的浓度与正常脂血症组相似，但E（-）C-III（-）颗粒分布在VLDL和IDL中多于LDL。与此相反，高胆固醇血症患者与血脂正常组的区别在于，不含apoE或apoC-III（E（-）C-III（-））的apoB脂蛋白浓度高2倍，主要是胆固醇含量高的LDL。尽管如此，血脂正常者和高胆固醇血症者都有含apoC-III的VLDL，占其总VLDL颗粒的68%和43%。E（+）C-III（-）颗粒是次要类型，在所有脂蛋白和患者组中占<10%的颗粒。因此，载脂蛋白C-Ⅲ的极低密度脂蛋白颗粒可能在确定高脂血症冠心病的高风险中发挥核心作用，但其在血脂正常者中的大量患病率也可能具有临床意义。
Apolipoprotein (apo) E and apoC-III concentrations in VLDL and LDL are associated with coronary heart disease. We studied the relationship between apoE and apoC-III and the abnormal concentrations and distribution of apoB lipoproteins in 10 hypercholesterolemic and 13 hypertriglyceridemic patients compared with 12 normolipidemic subjects (mean age, 45 years). Sixteen distinct types of apoB lipoprotein particles were separated by first using anti-apoE and anti-apoC-III immunoaffinity chromatography in sequence and then ultracentrifugation [light VLDL, dense VLDL, IDL, and LDL, with apoE with or without apoC-III (E(+)C-III(+), E(+)C-III(-)) or without apoE with or without apoC-III (E(-)C-III(+), E(-)C-III(-))]. The concentrations of VLDL particles with apoC-III (E(+)C-III(+), E(-)C-III(+)) were increased in the hypertriglyceridemic group compared with the hypercholesterolemic and normolipidemic groups. These particles were the most triglyceride rich of the particle types, and their triglyceride content was twice as high in hypertriglyceridemics compared with the other two groups. Hypertriglyceridemics had a similar concentration of total E(-)C-III(-) particles compared with normolipidemics, but the E(-)C-III(-) particles were distributed more to VLDL and IDL than to LDL. Hypercholesterolemics, in contrast, were distinguished from the normolipidemic group by 2-fold higher concentrations of apoB lipoproteins without apoE or apoC-III (E(-)C-III(-)), mainly LDL, which had high cholesterol content. Nonetheless, both normolipidemics and hypercholesterolemics had apoC-III-containing VLDL, which comprised 68% and 43% of their total VLDL particles. E(+)C-III(-) particles were a minor type, comprising <10% of particles in all lipoproteins and patient groups. Therefore, VLDL particles with apoC-III may play a central role in identifying the high risk of coronary heart disease in hypertriglyceridemia, but their substantial prevalence in normolipidemics may be of clinical significance as well.