Validation of Dried Blood Spots for Capturing Hepatitis C Virus Diversity for Genomic Surveillance.
Validation of Dried Blood Spots for Capturing Hepatitis C Virus Diversity for Genomic Surveillance.
复制标题
验证干血斑捕获丙型肝炎病毒多样性以进行基因组监测。
DOI：
10.1101/2023.07.06.23292160
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Allen,ToddM
中科院分区：
文献类型：
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作者：
Tully,DamienC;Power,KarenA;Sarette,Jacklyn;Stopka,ThomasJ;Friedmann,PeterD;Korthuis,PTodd;Cooper,Hannah;Young,AprilM;Seal,DavidW;Westergaard,RyanP;Allen,ToddM
干血斑 (DBS) 已成为丙型肝炎病毒 (HCV) 检测中传统静脉血的一种有前途的替代品。然而，人们对它们准确反映 HCV 遗传多样性的能力仍知之甚少。我们对来自两种常见亚型的配对血浆和 DBS 样本进行了深度测序和高级系统发育分析，以评估 DBS 对于基因组监测的适用性。结果表明，与血浆相比，DBS 捕获了相同的病毒多样性，没有观察到系统发育不一致。 DBS 准确反映病毒遗传多样性概况的能力表明，它可能是未来遏制 HCV 爆发的监测工作的一个有前途的途径。
Dried blood spots (DBS) have emerged as a promising alternative to traditional venous blood for hepatitis C virus (HCV) testing. However, their capacity to accurately reflect the genetic diversity of HCV remains poorly understood. We employed deep sequencing and advanced phylogenetic analyses on paired plasma and DBS samples from two common subtypes to evaluate the suitability of DBS for genomic surveillance. Results demonstrated that DBS captured equivalent viral diversity compared to plasma with no phylogenetic discordance observed. The ability of DBS to accurately reflect the profile of viral genetic diversity suggests it may be a promising avenue for future surveillance efforts to curb HCV outbreaks.