Global regulation of X chromosomal genes by the MSL complex in Drosophila melanogaster
Global regulation of X chromosomal genes by the MSL complex in Drosophila melanogaster
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DOI：
10.1101/gad.1343705
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发表时间：
2005-10-01
影响因子：
10.5
通讯作者：
Kuroda, MI
中科院分区：
文献类型：
--
作者：
Hamada, FN;Park, PJ;Kuroda, MI
一个长期存在的模型假设，果蝇的X染色体剂量补偿是通过上调单个雄性X染色体的两倍来实现的，但之前的数据不能排除另一种模型，在该模型中，雄性反染色体下调以平衡基因表达。为了区分这两种模型，我们使用RNA干扰来去除类雄性组织培养细胞中的雄性特异性致死(MSL)复合体。我们发现，X染色体上的许多基因表达下降，而常染色体上的表达基本没有变化。我们得出结论，MSL复合体的主要作用是上调男性X染色体。
A long-standing model postulates that X-chromosome dosage compensation in Drosophila occurs by twofold up-regulation of the single male X, but previous data cannot exclude an alternative model, in which male antosomes are down-regulated to balance gene expression. To distinguish between the two models, we used RNA interference to deplete Male-Specific Lethal (MSL) complexes from male-like tissue culture cells. We found that expression of many genes from the X chromosome decreased, while expression from the autosomes was largely unchanged. We conclude that the primary role of the MSL complex is to up-regulate the male X chromosome.