Bidirectional action of the Igf2r imprint control element on upstream and downstream imprinted genes
Bidirectional action of the Igf2r imprint control element on upstream and downstream imprinted genes
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DOI：
10.1101/gad.206201
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发表时间：
2001-09-15
影响因子：
10.5
通讯作者：
Barlow, DP
中科院分区：
文献类型：
--
作者：
Zwart, R;Sleutels, F;Barlow, DP
母体表达的Igf2r基因的印记由一个被称为区域2的内含子印记控制元件(ICE)控制，该元件包含非编码Air RNA的启动子，其转录产物以反义方向与沉默的父系Igf2r启动子重叠。这里描述了两个新的印记基因slc22a2和slc22a3，它们位于Igf2r的110和155kb 3‘，不与Air转录本重叠，但受Igf2r-ICE的调控，正如先前对Igf2r所示。这些结果确定了一组新的印迹基因，其通过区域2-ICE的双向作用而抑制，而不依赖于Air RNA的转录重叠。
Imprinting of the maternally-expressed Igf2r gene is controlled by an intronic imprint control element (ICE) known as Region2 that contains the promoter of the noncoding Air RNA, whose transcript overlaps the silenced paternal Igf2r promoter in an antisense orientation. Two novel imprinted genes, Slc22a2 and Slc22a3 are described here that lie 110 and 155 kb 3 ' to Igf2r and that are not overlapped by the Air transcript but are regulated by the Igf2r-ICE, as previously shown for Igf2r. These results identify a new cluster of imprinted genes whose repression by the bidirectional action of the Region2-ICE is independent of transcript overlap by the Air RNA.