THE DROSOPHILA ECR GENE ENCODES AN ECDYSONE RECEPTOR, A NEW MEMBER OF THE STEROID-RECEPTOR SUPERFAMILY
THE DROSOPHILA ECR GENE ENCODES AN ECDYSONE RECEPTOR, A NEW MEMBER OF THE STEROID-RECEPTOR SUPERFAMILY
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DOI：
10.1016/0092-8674(91)90572-g
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发表时间：
1991-10-04
期刊：
影响因子：
64.5
通讯作者：
HOGNESS, DS
中科院分区：
文献类型：
--
作者：
KOELLE, MR;TALBOT, WS;HOGNESS, DS
类固醇激素蜕皮激素触发果蝇组织发育的协调变化，导致变态。为了促进我们对控制这种组织反应的遗传调控等级的理解，我们分离并鉴定了一种新的类固醇受体同系物的基因ECR，并表明它编码一种蜕皮激素受体。首先，ECR蛋白与活性蜕皮激素结合，在抗原性上与以前在果蝇细胞系和组织提取物中观察到的蜕皮激素结合蛋白没有区别。第二，ECR蛋白与DNA结合具有高度的蜕皮激素反应元件特异性。第三，对蜕皮激素敏感的培养细胞表达ECR，而从它们衍生的蜕皮激素抵抗细胞缺乏ECR。通过转基因在这些耐药细胞中表达ECR，可以恢复它们对激素的反应能力。正如预期的那样，ECR是有核的，并在所有被检查的蜕皮激素靶组织中发现。此外，ECR基因在以蜕皮激素脉冲为标志的每个发育阶段都有表达。
The steroid hormone ecdysone triggers coordinate changes in Drosophila tissue development that result in metamorphosis. To advance our understanding of the genetic regulatory hierarchies controlling this tissue response, we have isolated and characterized a gene, EcR, for a new steroid receptor homolog and have shown that it encodes an ecdysone receptor. First, EcR protein binds active ecdysteroids and is antigenically indistinguishable from the ecdysone-binding protein previously observed in extracts of Drosophila cell lines and tissues. Second, EcR protein binds DNA with high specificity at ecdysone response elements. Third, ecdysone-responsive cultured cells express EcR, whereas ecdysone-resistant cells derived from them are deficient in EcR. Expression of EcR in such resistant cells by transfection restores their ability to respond to the hormone. As expected, EcR is nuclear and found in all ecdysone target tissues examined. Furthermore, the EcR gene is expressed at each developmental stage marked by a pulse of ecdysone.