Restoration of a normal reproductive lifespan after grafting of cryopreserved mouse ovaries
Restoration of a normal reproductive lifespan after grafting of cryopreserved mouse ovaries
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DOI：
10.1093/humrep/15.6.1300
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发表时间：
2000-06-01
影响因子：
6.1
通讯作者：
Whittingham, DG
中科院分区：
文献类型：
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作者：
Candy, CJ;Wood, MJ;Whittingham, DG
将来自10日龄C57 BL/6 J-Gpi-1（a）小鼠的新鲜和冷冻卵巢原位移植到卵巢切除的B6 CBF 1（纯合Gpi-1（B））受体中。受体与B6 CBF 1雄性交配。记录每窝的出生和大小。每个新生儿的葡萄糖磷酸异构酶的电泳变体进行了测定。13只新鲜卵巢和12只冷冻卵巢受体中分别有12只和10只可育。其中，10只（新鲜）和8只（冷冻）的窝只来自卵巢移植物，或来自移植物和天然卵巢。新鲜和冷冻移植受体的繁殖特性相似。新鲜（6.2窝）和冷冻（8.4窝）移植物受体的生殖寿命与与B6 CBF 1雄性交配的未手术C57 BL/6-Gpi-1（a）对照雌性（6.3窝）相似。移植卵巢受体（新鲜，5.4只幼仔/窝;冷冻，6.3只幼仔/窝）和C57 BL/6-Gpi-1（a）雌性（6.3只幼仔/窝）的窝仔数相似。结果表明，冷冻保存本身并不影响卵巢组织的长期生存能力，并为储存雌性生殖细胞提供了一种选择。这是第一个明确的证明，正常的生殖寿命可以恢复原位移植冷冻卵巢。
Fresh and frozen ovaries from 10 day old C57BL/6J-Gpi-1(a) mice were grafted orthotopically into ovariectomized B6CBF1 (homozygous Gpi-1(b)) recipients. The recipients were mated with B6CBF1 males. The birth and size of each litter was recorded. The electrophoretic variant of glucose phosphate isomerase was determined for each neonate. Twelve of 13 recipients of fresh ovary and 10 of 12 recipients of frozen ovary were fertile. Of these, 10 (fresh) and eight (frozen) had litters derived from the ovarian grafts only, or from the graft and native ovary. The breeding characteristics of recipients of fresh and frozen grafts were similar. The reproductive lifespan of the recipients of fresh (6.2 litters) and frozen (8.4 litters) grafts was similar to that of unoperated C57BL/6-Gpi-1(a) control females mated with B6CBF1 males (6.3 litters). Litter size was similar in recipients of grafted ovaries (fresh, 5.4 pups/litter; frozen, 6.3 pups/litter) and C57BL/6-Gpi-1(a) females (6.3 pups/litter). The results suggest that cryopreservation per se does not affect the long-term viability of ovarian tissue and provides an option for storing female germ cells. This is the first unequivocal demonstration that a normal reproductive lifespan can be restored by orthotopic grafting of frozen ovary.