Mutagenicity of L-azaserine for V79 cells in a pancreatic acinar cell-mediated mutagenesis assay.
Mutagenicity of L-azaserine for V79 cells in a pancreatic acinar cell-mediated mutagenesis assay.
复制标题
L-重氮丝氨酸在胰腺腺泡细胞介导的诱变测定中对 V79 细胞的诱变性。
DOI：
10.1097/00006676-198709000-00005
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Longnecker,DS
中科院分区：
文献类型：
--
作者：
Schaeffer,BK;Curphey,TJ;Longnecker,DS
使用V79细胞作为应答细胞系，在胰腺腺泡细胞介导的致突变试验中测定阿扎胞苷的致突变性。在与阿扎胞苷直接培养以及与大鼠和仓鼠胰腺腺泡细胞共培养中，V79细胞的突变频率增加。虽然在共培养中观察到的突变频率略高，但在共培养中由阿扎胞苷诱导的突变频率与直接培养中观察到的突变频率相比并没有显著增强。因此，在此类细胞介导的诱变试验中，阿扎胞苷不能用作阳性对照来监测腺泡细胞代谢水平。统计分析表明，仓鼠腺泡细胞共培养物更有效地增加阿扎胞苷的突变频率相比，大鼠腺泡细胞共培养物。大鼠腺泡细胞共培养物（而非大鼠腺泡细胞共培养物）以剂量-反应方式增加阿扎胞苷的致突变性。这些结果表明，阿扎胞苷可能是仓鼠和大鼠的胰腺致癌物。
The mutagenicity of azaserine was determined in a pancreatic acinar cell-mediated mutagenesis assay using V79 cells as the responder cell line. The mutation frequency of V79 cells was increased in direct culture with azaserine as well as in coculture with rat and hamster pancreatic acinar cells. Although slightly higher mutation frequencies were seen with coculture, the mutation frequency induced by azaserine in coculture was not significantly enhanced over that observed in direct culture. Thus, azaserine cannot be used as a positive control to monitor the level of acinar cell metabolism in such cell-mediated mutagenesis assays. Statistical analysis suggested that hamster acinar cell cocultures were more effective at increasing the mutation frequency of azaserine as compared to rat acinar cell cocultures. Hamster acinar cell cocultures, but not rat acinar cell cocultures, increased the mutagenicity of azaserine in a dose-response fashion. These results suggest that azaserine may be a pancreatic carcinogen for the hamster as well as the rat.