α- andβ-adrenergic control of thermogenin mRNA expression in brown adipose tissue
α- andβ-adrenergic control of thermogenin mRNA expression in brown adipose tissue
复制标题
棕色脂肪组织中产热素 mRNA 表达的 α- 和 β- 肾上腺素能控制
DOI：
10.1007/bf01114756
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发表时间：
1986
影响因子：
4
通讯作者：
B. Cannon
中科院分区：
文献类型：
--
作者：
A. Jacobsson;J. Nedergaard;B. Cannon
利用较早表征的cDNA克隆CIN-1（对应于编码棕色脂肪特异性“解偶联”蛋白产热素的mRNA序列），对不同生理和药理条件下小鼠棕色脂肪组织中产热素mRNA的量进行定量研究。结果发现，4小时的冷应激导致产热素mRNA的量增加7倍；注射去甲肾上腺素有显着但较小的效果。最值得注意的是，异丙肾上腺素（β-激动剂）和去氧肾上腺素（α-激动剂）本身没有作用，但一起注射时能够协同增加 mRNA 水平。在 4 小时冷应激小鼠中，去甲肾上腺素、异丙肾上腺素和霍乱毒素都可以进一步增强冷应激本身对 mRNA 水平的影响。胰岛素和糖皮质激素地塞米松均对mRNA水平有微弱的刺激作用。由此得出结论，细胞内cAMP水平的增加是产热素基因表达增加的必要且或许充分的刺激。然而，至少在体内条件下，这种增加需要刺激 α- 和 β- 肾上腺素能途径。
By the use of an earlier characterised cDNA clone, CIN-1, corresponding to a sequence of the mRNA coding for the brown-fat specific “uncoupling” protein, thermogenin, the amount of thermogenin mRNA found in the brown adipose tissue of mice was quantitatively investigated under different physiological and pharmacological conditions.It was found that a 4 hr cold stress led to a 7-fold increase in the amount of thermogenin mRNA; injection of norepinephrine had a significant but smaller effect. Most notably, isoprenaline (β-agonist) and phenylephrine (α-agonist) had in themselves no effect, but when injected together were able to increase the mRNA level synergistically. In 4 hr cold-stressed mice, norepinephrine, isoprenaline and cholera toxin could all further potentiate the effect of the cold stress itself on the mRNA level. Insulin and the glucocorticoid dexamethasone both had weak stimulatory effects on the mRNA level.It is concluded that an increase in intracellular cAMP levels is a necessary and perhaps sufficient stimulus for the increase in thermogenin gene expression. However, at least underin vivo conditions, this increase requires stimulation of both α- andβ-adrenergic pathways.
影响因子：
5
作者：
LANGIN, D;PORTILLO, MP;LAFONTAN, M
通讯作者：
LAFONTAN, M